Alterations of growth rate and gene expression levels of UPEC by antibiotics at sub-MIC
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
29327292
DOI
10.1007/s12223-017-0582-z
PII: 10.1007/s12223-017-0582-z
Knihovny.cz E-zdroje
- MeSH
- antibakteriální látky metabolismus farmakologie MeSH
- bakteriální proteiny genetika MeSH
- bakteriální toxiny genetika MeSH
- ciprofloxacin metabolismus farmakokinetika MeSH
- kombinace léků trimethoprim a sulfamethoxazol metabolismus farmakologie MeSH
- lidé MeSH
- membránové transportní proteiny genetika MeSH
- mikrobiální testy citlivosti MeSH
- nitrofurantoin metabolismus farmakologie MeSH
- proteiny z Escherichia coli genetika MeSH
- regulace genové exprese u bakterií fyziologie MeSH
- uropatogenní Escherichia coli účinky léků genetika růst a vývoj MeSH
- virulence genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
- bakteriální proteiny MeSH
- bakteriální toxiny MeSH
- ciprofloxacin MeSH
- cytotoxic necrotizing factor type 1 MeSH Prohlížeč
- FocA protein, E coli MeSH Prohlížeč
- kombinace léků trimethoprim a sulfamethoxazol MeSH
- membránové transportní proteiny MeSH
- nitrofurantoin MeSH
- proteiny z Escherichia coli MeSH
- USP protein, E coli MeSH Prohlížeč
- ymcE protein, E coli MeSH Prohlížeč
The host is the main environment for bacteria, and they also expose to many antibiotics during the treatment of infectious diseases in host body. In this study, it was aimed to investigate possible changes in growth rate and expression levels of three virulence genes (foc/foc, cnf1, and usp) in a uropathogenic E. coli standard strain within the presence of ciprofloxacin, nitrofurantoin, and trimethoprim-sulfamethoxazole. The UPEC C7 strain was grown on tryptic soy broth-TSB (control), TSB + ciprofloxacin, TSB + nitrofurantoin, and TSB + trimethoprim-sulfamethoxazole for determination of both growth rate and gene expression level. Antibiotics were added according to their sub-minimal inhibition concentrations. E-test was used to determine MIC values of antibiotics. Growth changes were measured in absorbance 600 nm during 24-h period. Total RNA isolations were performed after incubation for 24 h at 37 °C. Gene expression levels were determined by quantitative PCR. Tukey's post hoc test was used for statistical analysis. According to absorbance values, it has been shown that only ciprofloxacin and trimethoprim-sulfamethoxazole have lead significant decrease on growth rate. We also detected statistically significant differences in each gene expression levels for all antibiotics via relative quantification analysis. Fold changes in gene expression was found 0.65, 1.42, 0.23 for foc/foc gene; 0.01, 0.01, 2.84 for cnf1 gene; and 0.1, 0.01, 0.01 for usp gene in the presence of ciprofloxacin, nitrofurantoin, and trimethoprim/sulfamethoxazole, respectively. This investigation has shown that antibiotics can play a role as an environmental factor which may determine the pathogenicity of bacteria in vivo.
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