Chromatographic methods have become popular in clinical analysis in both routine and research laboratories. The purpose of this review article is to provide an overview of the current state of chromatographic methods, i.e., high-performance liquid chromatography (HPLC), gas chromatography (GC), and supercritical fluid chromatography (SFC), in clinical analysis. The aspects related to method sensitivity, selectivity, analysis time, and throughput have been discussed in detail. Adequate solutions to improve these features have also been presented. HPLC is the most widely used method among the chromatographic methods, whereas GC is dedicated to several specific applications, and SFC is used only marginally certainly due to its only recent comeback to the analytical scene. Based on the literature search, the application fields in clinical analysis are divided into the following groups: drugs, hormones, drugs of abuse, metabolomics, lipidomics, volatile organic compounds, biomarkers and endogenous compounds, proteomics, multi-analyte approches, and others. The important features of these applications have been emphasized.

Deparment of Analytical Chemistry Faculty of Pharmacy in Hradec Králové Charles University Heyrovského 1203 Hradec Králové Czech Republic

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Macrocyclic glycopeptide-based chiral selectors for enantioseparation in sub/supercritical fluid chromatography