Dendrimers, which are considered as one of the most promising tools in the field of nanobiotechnology due to their structural organization, showed a great potential in gene therapy, drug delivery, medical imaging and as antimicrobial and antiviral agents. This article is devoted to study interactions between new carbosilane-based metallodendrimers containing ruthenium and anti-cancer small interfering RNA (siRNA). Formation of complexes between anti-cancer siRNAs and Ru-based carbosilane dendrimers was evaluated by transmission electron microscopy, circular dichroism and fluorescence. The zeta-potential and the size of dendriplexes were determined by dynamic light scattering. The internalization of dendriplexes were estimated using HL-60 cells. Results show that ruthenium dendrimers associated with anticancer siRNA have the ability to deliver siRNA as non-viral vectors into the cancer cells. Moreover, dendrimers can protect siRNA against nuclease degradation. Nevertheless, further research need to be performed to examine the therapeutic potential of ruthenium dendrimers as well as dendrimers complexed with siRNA and anticancer drugs towards cancer cells.

Departamento Química Orgánica y Química Inorganica Universidad de Alcala de Henares Spain; Networking Research Center on Bioengineering Biomaterials and Nanomedicine Spain

Department of General Biophysics University of Lodz Pomorska 141 143 90 236 Lodz Poland

Department of Physics Faculty of Science J E Purkinje University in Ústí nad Labem Ústí nad Labem Czech Republic

Institute of Biophysics and Cell Engineering of NASB Akademicheskaja 27 Minsk 220072 Belarus

Laboratory of Microscopic Imaging and Specialized Biological Techniques Faculty of Biology and Environmental Protection University of Lodz Banacha12 16 90 237 Lodz Poland; Department of General Biophysics University of Lodz Pomorska 141 143 90 236 Lodz Poland

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