On the role of Nb-related sites of an oxidized β-TiNb alloy surface in its interaction with osteoblast-like MG-63 cells
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23827618
DOI
10.1016/j.msec.2012.12.073
PII: S0928-4931(12)00642-X
Knihovny.cz E-zdroje
- Klíčová slova
- Biocompatibility, Osteoblast-like MG-63 cells, Surface acidity, Surface oxide phase, β-TiNb alloy surface,
- MeSH
- buněčná adheze účinky léků MeSH
- fotoelektronová spektroskopie MeSH
- lidé MeSH
- mikroskopie elektronová rastrovací MeSH
- niob farmakologie MeSH
- osteoblasty cytologie účinky léků metabolismus MeSH
- oxidace-redukce účinky léků MeSH
- počet buněk MeSH
- povrchové vlastnosti účinky léků MeSH
- proliferace buněk účinky léků MeSH
- Ramanova spektroskopie MeSH
- slitiny farmakologie MeSH
- statická elektřina MeSH
- tvar buňky účinky léků MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- niob MeSH
- slitiny MeSH
- titanium-niobium alloy MeSH Prohlížeč
β-Stabilized titanium (Ti) alloys containing non-toxic elements, particularly niobium (Nb), are promising materials for the construction of bone implants. Their biocompatibility can be further increased by oxidation of their surface. Therefore, in this study, the adhesion, growth and viability of human osteoblast-like MG 63 cells in cultures on oxidized surfaces of a β-TiNb alloy were investigated and compared with the cell behavior on thermally oxidized Ti, i.e. a metal commonly used for constructing bone implants. Four experimental groups of samples were prepared: Ti or TiNb samples annealed to 600 °C for 60 min in a stream of dry air, and Ti and TiNb samples treated in Piranha solution prior to annealing. We found that on all TiNb-based samples, the cell population densities on days 1, 3 and 7 after seeding were higher than on the corresponding Ti-based samples. As revealed by XPS and Raman spectroscopy, and also by isoelectric point measurements, these results can be attributed to the presence of T-Nb2O5 oxide phase in the surface of the alloy sample, which decreased its negative zeta (ζ)-potential in comparison with zeta (ζ)-potential of the Ti sample at physiological pH. This effect was tentatively explained by the presence of positively charged defects acting as Lewis sites of the surface Nb2O5 phase. Piranha treatment slightly decreases the biocompatibility of the samples, which for the alloy samples may be explained by a decrease in the number of defective sites with this treatment. Thus, the presence of Nb and thermal oxidation of β-stabilized Ti alloys play a significant role in the increased biocompatibility of TiNb alloys.
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