On the role of Nb-related sites of an oxidized β-TiNb alloy surface in its interaction with osteoblast-like MG-63 cells
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23827618
DOI
10.1016/j.msec.2012.12.073
PII: S0928-4931(12)00642-X
Knihovny.cz E-resources
- Keywords
- Biocompatibility, Osteoblast-like MG-63 cells, Surface acidity, Surface oxide phase, β-TiNb alloy surface,
- MeSH
- Cell Adhesion drug effects MeSH
- Photoelectron Spectroscopy MeSH
- Humans MeSH
- Microscopy, Electron, Scanning MeSH
- Niobium pharmacology MeSH
- Osteoblasts cytology drug effects metabolism MeSH
- Oxidation-Reduction drug effects MeSH
- Cell Count MeSH
- Surface Properties drug effects MeSH
- Cell Proliferation drug effects MeSH
- Spectrum Analysis, Raman MeSH
- Alloys pharmacology MeSH
- Static Electricity MeSH
- Cell Shape drug effects MeSH
- Cell Survival drug effects MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Niobium MeSH
- Alloys MeSH
- titanium-niobium alloy MeSH Browser
β-Stabilized titanium (Ti) alloys containing non-toxic elements, particularly niobium (Nb), are promising materials for the construction of bone implants. Their biocompatibility can be further increased by oxidation of their surface. Therefore, in this study, the adhesion, growth and viability of human osteoblast-like MG 63 cells in cultures on oxidized surfaces of a β-TiNb alloy were investigated and compared with the cell behavior on thermally oxidized Ti, i.e. a metal commonly used for constructing bone implants. Four experimental groups of samples were prepared: Ti or TiNb samples annealed to 600 °C for 60 min in a stream of dry air, and Ti and TiNb samples treated in Piranha solution prior to annealing. We found that on all TiNb-based samples, the cell population densities on days 1, 3 and 7 after seeding were higher than on the corresponding Ti-based samples. As revealed by XPS and Raman spectroscopy, and also by isoelectric point measurements, these results can be attributed to the presence of T-Nb2O5 oxide phase in the surface of the alloy sample, which decreased its negative zeta (ζ)-potential in comparison with zeta (ζ)-potential of the Ti sample at physiological pH. This effect was tentatively explained by the presence of positively charged defects acting as Lewis sites of the surface Nb2O5 phase. Piranha treatment slightly decreases the biocompatibility of the samples, which for the alloy samples may be explained by a decrease in the number of defective sites with this treatment. Thus, the presence of Nb and thermal oxidation of β-stabilized Ti alloys play a significant role in the increased biocompatibility of TiNb alloys.
References provided by Crossref.org
The Photodynamic Properties and the Genotoxicity of Heat-Treated Silicalite-1 Films
Growth and potential damage of human bone-derived cells cultured on fresh and aged C60/Ti films
Growth and potential damage of human bone-derived cells on fresh and aged fullerene c60 films
