Nanoparticles (NPs) that form by self-assembly of amphiphilic poly(N-(2-hydroxypropyl)-methacrylamide) (pHPMA) copolymers bearing cholesterol side groups are potential drug carriers for solid tumor treatment. Here, we investigate their behavior in solutions of human serum albumin (HSA) in phosphate buffered saline. Mixed solutions of NPs, from polymer conjugates with or without the anticancer drug doxorubicin (Dox) bound to them, and HSA at concentrations up to the physiological value are characterized by synchrotron small-angle X-ray scattering and isothermal titration calorimetry. When Dox is absent, a small amount of HSA molecules bind to the cholesterol groups that form the core of the NPs by diffusing through the loose pHPMA shell or get caught in meshes formed by the pHPMA chains. These interactions are strongly hindered by the presence of Dox, which is distributed in the pHPMA shell, meaning that the delivery of Dox by the NPs in the human body is not affected by the presence of HSA.

European Molecular Biology Laboratory Hamburg Outstation c o Deutsches Elektronen Synchrotron Notkestr 85 22607 Hamburg Germany

Institute of Macromolecular Chemistry Czech Academy of Sciences v v i Heyrovsky Sq 2 162 06 Prague 6 Czech Republic

Technische Universität München Physik Department Physik weicher Materie James Franck Str 1 85748 Garching Germany

Citace poskytuje Crossref.org

HPMA Copolymer-Based Nanomedicines in Controlled Drug Delivery

Molecular Mechanisms of the Interactions of N-(2-Hydroxypropyl)methacrylamide Copolymers Designed for Cancer Therapy with Blood Plasma Proteins