Diversity of Entamoeba spp. in African great apes and humans: an insight from Illumina MiSeq high-throughput sequencing
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
29530647
DOI
10.1016/j.ijpara.2017.11.008
PII: S0020-7519(18)30055-9
Knihovny.cz E-zdroje
- Klíčová slova
- Central chimpanzee, Diversity, Entamoeba, Entamoeba histolytica, Humans, Metabarcoding, Mixed infections, Western lowland gorilla,
- MeSH
- entamébóza epidemiologie parazitologie veterinární MeSH
- Entamoeba * MeSH
- Gorilla gorilla parazitologie MeSH
- lidé MeSH
- nemoci lidoopů epidemiologie parazitologie MeSH
- Pan troglodytes parazitologie MeSH
- parazitární nemoci střev epidemiologie parazitologie veterinární MeSH
- vysoce účinné nukleotidové sekvenování * MeSH
- zachování přírodních zdrojů MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Afrika epidemiologie MeSH
Understanding the complex Entamoeba communities in the mammalian intestine has been, to date, complicated by the lack of a suitable approach for molecular detection of multiple variants co-occurring in mixed infections. Here, we report on the application of a high throughput sequencing approach based on partial 18S rDNA using the Illumina MiSeq platform. We describe, to our knowledge, for the first time, the Entamoeba communities in humans, free-ranging western lowland gorillas and central chimpanzees living in the Dja Faunal Reserve in Cameroon. We detected 36 Entamoeba haplotypes belonging to six haplotype clusters, containing haplotypes possessing high and low host specificity. Most of the detected haplotypes belonged to commensal Entamoeba, however, the pathogenic species (Entamoeba histolytica and Entamoeba nuttalli) were also detected. We observed that some Entamoeba haplotypes are shared between humans and other hosts, indicating their zoonotic potential. The findings are important not only for understanding the epidemiology of amoebiasis in humans in rural African localities, but also in the context of wild great ape conservation.
Institute of Parasitology University of Zurich Winterthurerstrasse 266a CH 8057 Zrich Switzerland
Institute of Vertebrate Biology Czech Academy of Sciences Květná 8 Brno 603 65 Czech Republic
Citace poskytuje Crossref.org
Soil-transmitted helminth infections in free-ranging non-human primates from Cameroon and Gabon
