Recent DNA-based studies have shown that the built environment is surprisingly rich in fungi. These indoor fungi - whether transient visitors or more persistent residents - may hold clues to the rising levels of human allergies and other medical and building-related health problems observed globally. The taxonomic identity of these fungi is crucial in such pursuits. Molecular identification of the built mycobiome is no trivial undertaking, however, given the large number of unidentified, misidentified, and technically compromised fungal sequences in public sequence databases. In addition, the sequence metadata required to make informed taxonomic decisions - such as country and host/substrate of collection - are often lacking even from reference and ex-type sequences. Here we report on a taxonomic annotation workshop (April 10-11, 2017) organized at the James Hutton Institute/University of Aberdeen (UK) to facilitate reproducible studies of the built mycobiome. The 32 participants went through public fungal ITS barcode sequences related to the built mycobiome for taxonomic and nomenclatural correctness, technical quality, and metadata availability. A total of 19,508 changes - including 4,783 name changes, 14,121 metadata annotations, and the removal of 99 technically compromised sequences - were implemented in the UNITE database for molecular identification of fungi (https://unite.ut.ee/) and shared with a range of other databases and downstream resources. Among the genera that saw the largest number of changes were Penicillium, Talaromyces, Cladosporium, Acremonium, and Alternaria, all of them of significant importance in both culture-based and culture-independent surveys of the built environment.

ATCC 10801 University Blvd Manassas Virginia 20110 USA

BCCM IHEM Scientific Institute of Public Health WIV ISP Juliette Wytsmanstraat 14 1050 Brussels Belgium

Biodiversity Ottawa Research and Development Centre Agriculture and Agri Food Canada Ottawa ON Canada K1A 0C6

Biosystematics Division ARC Plant Health and Protection P BagX134 Queenswood 0121 Pretoria South Africa

Bundesanstalt für Materialforschung und prüfung Department 4 Materials and Environment Unter den Eichen 87 12205 Berlin Germany

Dalla Lana School of Public Health University of Toronto Health Sciences Building 155 College Street 6th floor Toronto Ontario Canada M5T 3M7

Department of Biological and Environmental Sciences University of Gothenburg Box 463 405 30 Göteborg Sweden

Department of Biology University of Ottawa 30 Marie Curie Ottawa ON Canada K1N 6N5

Department of Botany Faculty of Science Charles University Prague Czech Republic

Department of Clinical Plant Science Faculty of Bioscience Hosei University 3 7 2 Kajino cho Koganei Tokyo Japan 184 8584

Department of Ecological and Biological Sciences University of Tuscia Viterbo 01100 Italy

Department of Ecology and Evolutionary Biology UC Irvine Irvine CA 92697 USA

Department of Experimental Limnology Leibniz Institute of Freshwater Ecology and Inland Fisheries Alte Fischerhuette 2 D 16775 Stechlin Germany

Department of Infectious Diseases Institute of Biomedicine The Sahlgrenska Academy University of Gothenburg Guldhedsgatan 10 SE 413 46 Gothenburg Sweden

Department of Plant Pathology and Microbiology and Institute of Integrative Genome Biology University of California Riverside Riverside 92501 CA USA

Evangelisches Schulzentrum Martinschule Max Planck Str 7 17491 Greifswald Germany

Gothenburg Global Biodiversity Centre Box 461 SE 405 30 Göteborg Sweden

Institute of Botany Nature Research Centre Žaliųjų ežerų Str 49 08406 Vilnius Lithuania

Institute of Microbiology Academy of Sciences of the Czech Republic v v i Prague Czech Republic

Leibniz Institute DSMZ German Collection of Microorganisms and Cell Cultures Inhoffenstrasse 7 B 38124 Braunschweig Germany

Plant and Microbial Biology University of California 94720 Berkeley California USA

School of Biological Sciences Seoul National University Seoul Republic of Korea

Sporometrics 219 Dufferin Street Suite 20C Toronto Ontario Canada M6K 1Y9

Steinbeis Innovationszentrum Organismische Mykologie und Mikrobiologie Vor dem Kreuzberg 17 72070 Tübingen Germany

Sydney Medical School Westmead Hospital Molecular Mycology Research Laboratory Centre for Infectious Diseases and Microbiology Sydney Australia

The James Hutton Institute and University of Aberdeen Aberdeen United Kingdom

UCIBIO REQUIMTE DCV Faculdade de Ciências e Tecnologia Universidade Nova de Lisboa 2829 516 Caparica Portugal

Université catholique de Louvain Earth and Life Institute Applied Microbiology BCCM MUCL Louvain la Neuve Belgium

University of Sydney Marie Bashir Institute for Infectious Diseases and Biosecurity Sydney Australia

University of Tartu Tartu Estonia

Westerdijk Fungal Biodiversity Institute Uppsalalaan 8 3584 CT Utrecht The Netherlands

Westmead Institute for Medical Research Westmead Australia

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