The contact assay measuring the inhibition of Arthrobacter globiformis dehydrogenase activity as an endpoint to evaluate the toxicity of solid samples was tested in an international ring-test to validate its performance for ISO standardization (ISO/CD 18187). This work reports the results of the ring-test involving 9 laboratories from six countries. At least 8 valid data sets were obtained for each sample and more than three quarters of the participants attained the validity criteria defined in the standard. The coefficient of variation within (CVr) and between (CVR) laboratories was generally on average <15% and <30% for negative and positive controls, respectively. Regarding solid samples, the laboratories provided a similar ranking of the samples based on their toxicity, despite some variation in the LOEC values. The logarithmic within-lab standard deviation <0.50 for soils and <0.25 for wastes evidenced a good repeatability. The between-lab variability assessed by a CVR <30%, minimum-maximum factor <4 and a reproducibility standard deviation (SDR) <0.13 for a great part of the solid samples, confirmed the test reproducibility. Overall, this assay proved to be robust, sensitive and feasible for routine use towards the quality assessment of soils and wastes.

Agroécologie AgroSup Dijon INRA Univ Bourgogne Franche Comté F 21000 Dijon France

Analytical Chemistry Physical Chemistry and Chemical Engineering Department Universidad de Alcalá 28871 Madrid Spain

Central Institute for Supervising and Testing in Agriculture Department of Microbiology and Biochemistry Hroznová 2 Brno CZ 656 06 Czech Republic

CSIRO Land and Water Reception Gate 4 Waite Road SA 5064 Urrbrae Australia

Departamento de Biologia and Centro de Estudos do Ambiente e do Mar Universidade de Aveiro Campus Universitário de Santiago 3810 193 Aveiro Portugal

ECT Oekotoxikologie GmbH Böttgerstraße 2 14 D 65439 Flörsheim a M Germany

Goethe University Frankfurt am Main Institute for Ecology Evolution and Diversity Department Aquatic Ecotoxicology Frankfurt am Main Germany

INERIS Parc Technologique Alata Verneuil en Halatte France

LUBW Landesanstalt für Umwelt Messungen und Naturschutz Baden Württemberg Griesbachstr 1 76185 Karlsruhe Germany

Research centre for toxic compounds in the environment Faculty of Science Masaryk University Kamenice 753 5 Brno 625 00 Czech Republic

SATT Grand Est Welience Agro Environnement F 21000 Dijon France

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