Metal-induced oxidative stress in terrestrial macrolichens
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
29627607
DOI
10.1016/j.chemosphere.2018.03.112
PII: S0045-6535(18)30529-0
Knihovny.cz E-resources
- Keywords
- Antioxidants, Bioaccumulation, Fluorescence microscopy, Heavy metals, Oxidative stress, Reactive oxygen species (ROS),
- MeSH
- Antioxidants metabolism MeSH
- Buthionine Sulfoximine MeSH
- Chromium toxicity MeSH
- Phytochelatins metabolism MeSH
- Glutathione metabolism MeSH
- Metals toxicity MeSH
- Ascorbic Acid metabolism MeSH
- Environmental Pollutants toxicity MeSH
- Lichens drug effects metabolism physiology MeSH
- Copper metabolism MeSH
- Oxidative Stress physiology MeSH
- Hydrogen Peroxide metabolism MeSH
- Lipid Peroxidation MeSH
- Reactive Oxygen Species metabolism MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antioxidants MeSH
- Buthionine Sulfoximine MeSH
- Chromium MeSH
- Phytochelatins MeSH
- Glutathione MeSH
- Metals MeSH
- Ascorbic Acid MeSH
- Environmental Pollutants MeSH
- Copper MeSH
- Hydrogen Peroxide MeSH
- Reactive Oxygen Species MeSH
Short-term (24 h) responses of Cladonia arbuscula subsp. mitis and Cladonia furcata to copper (CuII) or chromium (CrIII) excess (10 or 100 μM) were compared. C. arbuscula accumulated more Cu and Cr at higher metal doses but both species revealed depletion of K and/or Ca amount. Not only Cu but also Cr typically elevated reactive oxygen species (ROS) formation (fluorescence microscopy detection of total ROS and hydrogen peroxide) and depleted nitric oxide (NO) signal, with Cu showing more negative impact on lipid peroxidation (BODIPY 581/591 C11 staining reagent). Metals and staining reagents also affected anatomical responses and photobiont/mycobiont visibility. Principally different impact of Cu and Cr was observed at antioxidative metabolites level, indicating various ways of metal-induced ROS removal and/or metal chelation: Cu strongly depleted glutathione (GSH) and stimulated phytochelatin 2 (PC2) content while ascorbic acid accumulation was depleted by Cu and stimulated by Cr. Subsequent experiment with GSH biosynthetic inhibitor (buthionine sulfoximine, BSO) revealed that 48 h of exposure is needed to deplete GSH and BSO-induced depletion of GSH and PC2 amounts under Cu or Cr excess elevated ROS but depleted NO. These data suggest close relations between thiols, NO and appearance of oxidative stress (ROS generation) under metallic stress also in lichens.
Department of Biology University of Trnava Priemyselná 4 918 43 Trnava Slovak Republic
Department of Plant Physiology Maria Curie Skłodowska University Akademicka 19 20 033 Lublin Poland
References provided by Crossref.org
Mercury Content and Amelioration of Its Toxicity by Nitric Oxide in Lichens
