Turning Off Transcription with Bacterial RNA Polymerase through CuAAC Click Reactions of DNA Containing 5-Ethynyluracil
Language English Country Germany Media print-electronic
Document type Journal Article
- Keywords
- DNA, RNA polymerase, epigenetics, nucleotides, transcription,
- MeSH
- Alkynes chemistry MeSH
- Azides chemistry MeSH
- RNA, Bacterial chemistry metabolism MeSH
- Cycloaddition Reaction MeSH
- DNA-Directed RNA Polymerases chemistry metabolism MeSH
- DNA chemistry MeSH
- Escherichia coli chemistry MeSH
- Catalysis MeSH
- Coumarins chemistry MeSH
- Copper MeSH
- Click Chemistry MeSH
- Uracil analogs & derivatives MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- 3-azido-7-hydroxycoumarin MeSH Browser
- Alkynes MeSH
- Azides MeSH
- RNA, Bacterial MeSH
- DNA-Directed RNA Polymerases MeSH
- DNA MeSH
- eniluracil MeSH Browser
- Coumarins MeSH
- Copper MeSH
- Uracil MeSH
Copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction in the major groove of DNA containing 5-ethynyluracil (UE ) with azides was used for turning off sequence-specific protein-DNA interactions. The concept was first demonstrated on switching off cleavage of short modified DNA by restriction endonuclease BamHI-HF. Finally, DNA template containing UE was used for in vitro transcription with E. coli RNA polymerase and the transcription was turned off by CuAAC with 3-azidopropane-1,2-diol or 3-azido-7-hydroxycoumarin.
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