Antibiotic resistance profiles of coagulase-positive and coagulase-negative staphylococci from pit latrine fecal sludge in a peri-urban South African community
Language English Country United States Media print-electronic
Document type Comparative Study, Journal Article
PubMed
29663126
DOI
10.1007/s12223-018-0605-4
PII: 10.1007/s12223-018-0605-4
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Genes, Bacterial genetics MeSH
- Bacterial Proteins metabolism MeSH
- DNA, Bacterial genetics MeSH
- Feces microbiology MeSH
- Coagulase metabolism MeSH
- Microbial Sensitivity Tests MeSH
- Microbial Viability drug effects MeSH
- Drug Resistance, Multiple, Bacterial * MeSH
- Sewage microbiology MeSH
- Staphylococcus drug effects enzymology genetics isolation & purification MeSH
- Toilet Facilities * MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Geographicals
- South Africa MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Bacterial Proteins MeSH
- DNA, Bacterial MeSH
- Coagulase MeSH
- Sewage MeSH
The aim of this study was to assess pit latrine samples from a peri-urban community in KwaZulu-Natal (South Africa) for the presence of multidrug-resistant (MDR) Staphylococcus spp. Standard procedures were used to isolate Staphylococcus spp. from pit latrine fecal sludge samples, with confirmation at genus level by polymerase chain reaction (PCR). Sixty-eight randomly selected pit latrine Staphylococcus spp. isolates were further characterized by using established disk diffusion procedures. An average Staphylococcus spp. count of 2.1 × 105 CFU per g fecal material was established using two randomly selected pit latrine samples. Of the 68-selected Staphylococcus spp. pit latrine isolates, 49% were identified as coagulase positive, 51% as coagulase negative and 65% (12 coagulase positive, 32 coagulase negative isolates) were categorized as MDR. The majority (66/68) of Staphylococcus spp. isolates displayed resistance to fusidic acid while only 5/68 isolates displayed resistance to chloramphenicol. The pit latrine samples analyzed in this study are a source of MDR Staphylococcus spp., highlighting the need for proper hygiene and sanitation regimes in rural communities using these facilities.
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