Mitochondrial genome modulates myocardial Akt/Glut/HK salvage pathway in spontaneously hypertensive rats adapted to chronic hypoxia
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Akt pathway, SHR strains, cardioprotection, hypoxia, mitochondrial genome,
- MeSH
- druhová specificita MeSH
- faktor 1 indukovatelný hypoxií genetika metabolismus MeSH
- fyziologická adaptace genetika MeSH
- genom mitochondriální genetika MeSH
- hexokinasa genetika metabolismus MeSH
- hypertenze genetika MeSH
- hypoxie * MeSH
- myokard metabolismus MeSH
- potkani inbrední BN MeSH
- potkani inbrední SHR MeSH
- proteiny usnadňující transport glukosy genetika metabolismus MeSH
- protoonkogenní proteiny c-akt genetika metabolismus MeSH
- signální transdukce genetika MeSH
- srdeční mitochondrie genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- faktor 1 indukovatelný hypoxií MeSH
- hexokinasa MeSH
- proteiny usnadňující transport glukosy MeSH
- protoonkogenní proteiny c-akt MeSH
Recently we have shown that adaptation to continuous normobaric hypoxia (CNH) decreases myocardial ischemia/reperfusion injury in spontaneously hypertensive rats (SHR) and in a conplastic strain (SHR-mtBN). The protective effect was stronger in the latter group characterized by a selective replacement of the SHR mitochondrial genome with that of a more ischemia-resistant Brown Norway strain. The aim of the present study was to examine the possible involvement of the hypoxia inducible factor (HIF)-dependent pathway of the protein kinase B/glucose transporters/hexokinase (Akt/GLUT/HK) in this mitochondrial genome-related difference of the cardioprotective phenotype. Adult male rats were exposed for 3 wk to CNH ([Formula: see text] 0.1). The expression of dominant isoforms of Akt, GLUT, and HK in left ventricular myocardium was determined by real-time RT-PCR and Western blotting. Subcellular localization of GLUTs was assessed by quantitative immunofluorescence. Whereas adaptation to hypoxia markedly upregulated protein expression of HK2, GLUT1, and GLUT4 in both rat strains, Akt2 protein level was significantly increased in SHR-mtBN only. Interestingly, a higher content of HK2 was revealed in the sarcoplasmic reticulum-enriched fraction in SHR-mtBN after CNH. The increased activity of HK determined in the mitochondrial fraction after CNH in both strains suggested an increase of HK association with mitochondria. Interestingly, HIF1a mRNA increased and HIF2a mRNA decreased after CNH, the former effect being more pronounced in SHR-mtBN than in SHR. Pleiotropic effects of upregulated Akt2 along with HK translocation to mitochondria and mitochondria-associated membranes can potentially contribute to a stronger CNH-afforded cardioprotection in SHR-mtBN compared with progenitor SHR.
Department of Physiology Faculty of Science Charles University Prague Czech Republic
Institute of Physiology of the Czech Academy of Sciences Prague Czech Republic
Citace poskytuje Crossref.org
Sixty Years of Heart Research in the Institute of Physiology of the Czech Academy of Sciences
The involvement of protein kinases in the cardioprotective effect of chronic hypoxia
