Advances in the cellular structural biology of nucleic acids
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem, přehledy
PubMed
29679394
DOI
10.1002/1873-3468.13054
Knihovny.cz E-zdroje
- Klíčová slova
- DNA, RNA, in-cell EPR, in-cell NMR, in-cell chemical probing, in-cell single-molecule FRET,
- MeSH
- analýza jednotlivých buněk MeSH
- buňky chemie MeSH
- elektronová paramagnetická rezonance MeSH
- konformace nukleové kyseliny MeSH
- lidé MeSH
- magnetická rezonanční spektroskopie MeSH
- molekulární modely MeSH
- nukleové kyseliny chemie metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Názvy látek
- nukleové kyseliny MeSH
Conventional biophysical and chemical biology approaches for delineating relationships between the structure and biological function of nucleic acids (NAs) abstract NAs from their native biological context. However, cumulative experimental observations have revealed that the structure, dynamics and interactions of NAs might be strongly influenced by a broad spectrum of specific and nonspecific physical-chemical environmental factors. This consideration has recently sparked interest in the development of novel tools for structural characterization of NAs in the native cellular context. Here, we review the individual methods currently being employed for structural characterization of NA structure in a native cellular environment with a focus on recent advances and developments in the emerging fields of in-cell NMR and electron paramagnetic resonance spectroscopy and in-cell single-molecule FRET of NAs.
Central European Institute of Technology Masaryk University Brno Czech Republic
Faculty of Science University of South Bohemia Ceske Budejovice Czech Republic
Institute of Biophysics Academy of Science of the Czech Republic Brno Czech Republic
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