Advances in the cellular structural biology of nucleic acids
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
- Keywords
- DNA, RNA, in-cell EPR, in-cell NMR, in-cell chemical probing, in-cell single-molecule FRET,
- MeSH
- Single-Cell Analysis MeSH
- Cells chemistry MeSH
- Electron Spin Resonance Spectroscopy MeSH
- Nucleic Acid Conformation MeSH
- Humans MeSH
- Magnetic Resonance Spectroscopy MeSH
- Models, Molecular MeSH
- Nucleic Acids chemistry metabolism MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- Nucleic Acids MeSH
Conventional biophysical and chemical biology approaches for delineating relationships between the structure and biological function of nucleic acids (NAs) abstract NAs from their native biological context. However, cumulative experimental observations have revealed that the structure, dynamics and interactions of NAs might be strongly influenced by a broad spectrum of specific and nonspecific physical-chemical environmental factors. This consideration has recently sparked interest in the development of novel tools for structural characterization of NAs in the native cellular context. Here, we review the individual methods currently being employed for structural characterization of NA structure in a native cellular environment with a focus on recent advances and developments in the emerging fields of in-cell NMR and electron paramagnetic resonance spectroscopy and in-cell single-molecule FRET of NAs.
Central European Institute of Technology Masaryk University Brno Czech Republic
Faculty of Science University of South Bohemia Ceske Budejovice Czech Republic
Institute of Biophysics Academy of Science of the Czech Republic Brno Czech Republic
References provided by Crossref.org
