Molecular Evidence of Toxoplasma gondii, Neospora caninum, and Encephalitozoon cuniculi in Red Foxes ( Vulpes vulpes)
Language English Country United States Media print-electronic
Document type Journal Article
PubMed
29733765
DOI
10.7589/2017-09-240
Knihovny.cz E-resources
- Keywords
- Carnivores, PCR, encephalitozoonosis, neosporosis, toxoplasmosis,
- MeSH
- Animals, Wild MeSH
- Encephalitozoon cuniculi genetics isolation & purification MeSH
- Encephalitozoonosis epidemiology microbiology veterinary MeSH
- Genotype MeSH
- Coccidiosis epidemiology parasitology veterinary MeSH
- Foxes parasitology MeSH
- Neospora genetics isolation & purification MeSH
- Toxoplasma genetics isolation & purification MeSH
- Toxoplasmosis, Animal epidemiology parasitology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
Toxoplasma gondii, Neospora caninum, and Encephalitozoon cuniculi are important infectious agents, with T. gondii and E. cuniculi having zoonotic potential. There are two main clonal lineages (types I and II) of T. gondii in Europe, but little is known about genotypes of T. gondii in wild animals. The aim of our study was molecular detection of these three pathogens in tissues of wild red foxes ( Vulpes vulpes) from the Czech Republic. Using PCR (B1 gene), we detected T. gondii in 10% of the animals that we tested ( n=100); N. caninum and E. cuniculi were not detected. The T. gondii samples were genotyped by single multiplex PCR assay with 15 microsatellite markers. Five samples were successfully genotyped as genotype II, a unique finding for T. gondii isolated from red foxes from the Czech Republic.
INSERM UMR S 1094 Neuroépidémiologie Tropicale Université de Limoges 87025 Limoges France
State Veterinary Institute Prague Sídlištní 136 24 165 03 Prague 6 Czech Republic
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