Profiling system for skin kallikrein proteolysis applied in gene-deficient mouse models
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
29885275
DOI
10.1515/hsz-2018-0116
PII: /j/bchm.just-accepted/hsz-2018-0116/hsz-2018-0116.xml
Knihovny.cz E-resources
- Keywords
- inhibitors, kallikreins, mouse model, proteolysis, skin, substrates,
- MeSH
- Kallikreins deficiency genetics metabolism MeSH
- Skin metabolism MeSH
- Humans MeSH
- Disease Models, Animal * MeSH
- Mice, Knockout MeSH
- Mice MeSH
- Proteolysis * MeSH
- Gene Expression Profiling MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Kallikreins MeSH
Kallikrein-related proteases (KLKs) play a critical role in epidermis physiology and have been implicated in skin pathologies such as Netherton syndrome. The contribution of individual KLKs to skin proteolysis is poorly understood. Monitoring of their activities in skin proteome is hampered by overlapping substrate specificities, and there is a need for novel assays. Here, we present a platform of selective and sensitive fluorogenic substrates and inhibitors for profiling KLK5, KLK7 and KLK14. These chemical tools were evaluated using recombinant KLKs and tissue from a unique set of mice deficient in eight combinations of KLKs and their natural regulator LEKTI.
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