Immunological examination of peripheral blood in patients with colorectal cancer compared to healthy controls
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články
- Klíčová slova
- CD3+ lymphocytes, CD8+ lymphocytes, TGF-beta, VEGF, colorectal cancer,
- MeSH
- adenokarcinom imunologie MeSH
- B-lymfocyty imunologie MeSH
- buněčná imunita MeSH
- buňky NK imunologie MeSH
- CD4-pozitivní T-lymfocyty imunologie MeSH
- CD8-pozitivní T-lymfocyty imunologie MeSH
- kolorektální nádory imunologie MeSH
- krevní oběh MeSH
- lidé středního věku MeSH
- lidé MeSH
- počet lymfocytů MeSH
- staging nádorů MeSH
- transformující růstový faktor beta1 krev MeSH
- vaskulární endoteliální růstový faktor A krev MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- transformující růstový faktor beta1 MeSH
- vaskulární endoteliální růstový faktor A MeSH
BACKGROUND: The objective of this study was to investigate serum levels of immunosuppressive cytokines TGF beta 1 and VEGF and count of immune cells in peripheral blood in stage II and III colorectal cancer patients. METHODS: Blood samples were collected from 22 colorectal patients and 25 healthy controls before the start of treatment. All patients were examined by a clinical immunologist to exclude patients with immune disorders and autoimmune diseases. TGF beta 1 and VEGF were measured by ELISA, and anti-tumor cellular immunity cells (CD4, CD8, B cells, NK cells) were measured by flow cytometry. RESULTS: TGF beta 1 and VEGF plasma levels were significantly increased in stage II and III colorectal patients compared with control group (both p < 0.0001). A decrease in the cellular immunity was shown in the absolute numbers of cytotoxic T lymphocytes (CD8+ ; p = 0.0240), helper T lymphocytes (CD4+ ; p = 0.0019), and natural killer cells (CD16 + CD56+; p < 0.0001) in both stage II and stage III patients. On the contrary, B lymphocyte (CD19+) serum levels were increased in colon cancer patients (p < 0.0001) compared to the control group. CONCLUSIONS: Our results show peripheral blood levels of TGF beta and VEGF were significantly increased in colorectal patients and changes in cellular anticancer immunity in comparison to control group. These results will be compared with results from Immunoscore.
c Department of Pathology 1st Faculty of Medicine Charles University Praha Czech Republic
Department of Oncology 1st Faculty of Medicine Charles University Prague Czech Republic
Citace poskytuje Crossref.org
