Immunological examination of peripheral blood in patients with colorectal cancer compared to healthy controls
Language English Country England, Great Britain Media print-electronic
Document type Journal Article
- Keywords
- CD3+ lymphocytes, CD8+ lymphocytes, TGF-beta, VEGF, colorectal cancer,
- MeSH
- Adenocarcinoma immunology MeSH
- B-Lymphocytes immunology MeSH
- Immunity, Cellular MeSH
- Killer Cells, Natural immunology MeSH
- CD4-Positive T-Lymphocytes immunology MeSH
- CD8-Positive T-Lymphocytes immunology MeSH
- Colorectal Neoplasms immunology MeSH
- Blood Circulation MeSH
- Middle Aged MeSH
- Humans MeSH
- Lymphocyte Count MeSH
- Neoplasm Staging MeSH
- Transforming Growth Factor beta1 blood MeSH
- Vascular Endothelial Growth Factor A blood MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Transforming Growth Factor beta1 MeSH
- Vascular Endothelial Growth Factor A MeSH
BACKGROUND: The objective of this study was to investigate serum levels of immunosuppressive cytokines TGF beta 1 and VEGF and count of immune cells in peripheral blood in stage II and III colorectal cancer patients. METHODS: Blood samples were collected from 22 colorectal patients and 25 healthy controls before the start of treatment. All patients were examined by a clinical immunologist to exclude patients with immune disorders and autoimmune diseases. TGF beta 1 and VEGF were measured by ELISA, and anti-tumor cellular immunity cells (CD4, CD8, B cells, NK cells) were measured by flow cytometry. RESULTS: TGF beta 1 and VEGF plasma levels were significantly increased in stage II and III colorectal patients compared with control group (both p < 0.0001). A decrease in the cellular immunity was shown in the absolute numbers of cytotoxic T lymphocytes (CD8+ ; p = 0.0240), helper T lymphocytes (CD4+ ; p = 0.0019), and natural killer cells (CD16 + CD56+; p < 0.0001) in both stage II and stage III patients. On the contrary, B lymphocyte (CD19+) serum levels were increased in colon cancer patients (p < 0.0001) compared to the control group. CONCLUSIONS: Our results show peripheral blood levels of TGF beta and VEGF were significantly increased in colorectal patients and changes in cellular anticancer immunity in comparison to control group. These results will be compared with results from Immunoscore.
c Department of Pathology 1st Faculty of Medicine Charles University Praha Czech Republic
Department of Oncology 1st Faculty of Medicine Charles University Prague Czech Republic
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