Fullerene as a doxorubicin nanotransporter for targeted breast cancer therapy: Capillary electrophoresis analysis
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Breast tumors, Doxorubicin, Drug delivery systems, Fullerene, Nanomedicine,
- MeSH
- Doxorubicin therapeutic use MeSH
- Electrophoresis, Capillary MeSH
- Fluorescent Dyes chemistry MeSH
- Fullerenes chemistry MeSH
- Hydrogen-Ion Concentration MeSH
- Humans MeSH
- MCF-7 Cells MeSH
- Breast Neoplasms drug therapy MeSH
- Nanoparticles chemistry MeSH
- Drug Carriers chemistry MeSH
- Optical Imaging MeSH
- Surface Properties MeSH
- Antibiotics, Antineoplastic therapeutic use MeSH
- Drug Liberation MeSH
- Particle Size MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Doxorubicin MeSH
- Fluorescent Dyes MeSH
- Fullerenes MeSH
- Drug Carriers MeSH
- Antibiotics, Antineoplastic MeSH
The clinical use of doxorubicin (DOX) is limited by dose-related cardiomyopathy, which becomes more prevalent with increasing cumulative doses of the drug. Complexes of fullerene with DOX were designed and studied using biophysical methods. The ability of DOX to release from fullerene at different pHs was analyzed. It has been shown that the size of the fullerene-DOX complexes was ∼280 nm. The zeta potential for fullerene was -30 mV, for DOX -8 mV, and for fullerene-DOX conjugates -24 mV. Drug release was studied by CE with LIF detection. When fullerene-DOX conjugates were separated in a pH 7.5 buffer, 43% of all DOX signals were derived from free DOX, with the signal increasing as pH decreased. At pH 5.25, all DOX had been released and 100% of the signal was derived from free DOX. The release of DOX from complexes with fullerene at lower pH can be used in targeted antineoplastic therapy, resulting in lower toxicity for less acidic non-target tissue.
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