Advanced microscopy methods for bioimaging of mitotic microtubules in plants
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
29957201
DOI
10.1016/bs.mcb.2018.03.019
PII: S0091-679X(18)30019-0
Knihovny.cz E-zdroje
- Klíčová slova
- Arabidopsis, Light-sheet microscopy, Medicago, Microtubules, Mitosis, Phragmoplast, Plant, Preprophase band, Spindle, Superresolution microscopy,
- MeSH
- Arabidopsis metabolismus fyziologie MeSH
- mikroskopie metody MeSH
- mikrotubuly fyziologie MeSH
- mitóza fyziologie MeSH
- proteiny asociované s mikrotubuly metabolismus MeSH
- proteiny huseníčku metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- proteiny asociované s mikrotubuly MeSH
- proteiny huseníčku MeSH
Mitotic cell division in plants is a dynamic process playing a key role in plant morphogenesis, growth, and development. Since progress of mitosis is highly sensitive to external stresses, documentation of mitotic cell division in living plants requires fast and gentle live-cell imaging microscopy methods and suitable sample preparation procedures. This chapter describes, both theoretically and practically, currently used advanced microscopy methods for the live-cell visualization of the entire process of plant mitosis. These methods include microscopy modalities based on spinning disk, Airyscan confocal laser scanning, structured illumination, and light-sheet bioimaging of tissues or whole plant organs with diverse spatiotemporal resolution. Examples are provided from studies of mitotic cell division using microtubule molecular markers in the model plant Arabidopsis thaliana, and from deep imaging of mitotic microtubules in robust plant samples, such as legume crop species Medicago sativa.
Citace poskytuje Crossref.org
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