AIM: The objective of this work was to test the effects of adding dried Chicory root and White lupine food on small bowel morphology and compare it to a standard commercial diet. BACKGROUND: Various commercial gluten-free products, gluten-free raw materials and gluten-free plants are this time available on the food market, but there are still not enough information about their effect on the small bowel morphology. METHODS: Altogether thirty rabbits were used in this study. The control diet (C) contained common feed components. The first experimental diet (E1) contained (per kg) 60 g of dried chicory roots instead of barley, whereas the second experimental diet (E2) was based on white lupine seeds (cv. Amiga; 120 g per kg diet) instead of the soybean meal used in the control diet. The experiment started when the rabbits were 34-days old and lasted until they were 55-days old. At the end, one jejunal small bowel tissue was sampled, and both the heights and depths of the villi and crypts were measured. RESULTS: The highest villi were measured in the E1 (598.99 µm) group, mean in the C (590.30 µm) group and the lowest were in the E2 (563.74 µm) group. The most intense mucin villous positivity was observed in the E2 group, followed by the E1 group, and the weakest positivity was found in the visible C group. CONCLUSION: Chicory root has practical uses in gluten-free industries.

Department of Biology Faculty of Education Selye Janos University Komarno Slovak Republic

Laboratory of Veterinary Histopathology in Komarno Slovak Republic

Physiology of Nutrition and Quality of Animal Product Institute of Animal Science Prague Czech Republic

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Parzanese I, Quhajaja D, Patrinicola F, Aralica M, Chiriva-Internati M, Stifter S, et al. Celiac disease: From pathophysiology to treatment. World J Gastrointest Pathophysiol. 2017;8:27–38. PubMed PMC

Izadi F, Rezaei Tavirani M, Honarkar Z, Rostami-Nejad M. Celiac disease and hepatitis C relationships in transcriptional regulatory networks. Gastroenterol Hepatol Bed Bench. 2017;10:303–10. PubMed PMC

Mlynekova Z, Chrenkova M, Formelova Z. Cereals and legumes in nutrition of people with celiac disease. Int J Celiac Dis. 2014;2:105–9.

Oza SS, Akbari M, Kelly CP, Hansen J, Theethira T, Tariq S, et al. Socioeconomic risk factors for celiac disease burden and symptoms. J Clin Gastroenterol. 2016;50:307–12. PubMed

Ciska E, Drabinska N, Narwojsz A, Honke J. Stability of glucosinolates and glucosinolate degradation products during storage of boiled white cabbage. Food Chem. 2016;203:340–7. PubMed

OShea NA, Arendt E, Gallagher E. State of the art in gluten-free research. J Food Sci. 2014;79:R1067–76. PubMed

Scarnato L, Serrazanetti DI, Aloisi I, Montanari C, Del Duca S, Lanciotti R. Combination of transglutaminase and sourdough on gluten-free flours to improve dough structure. Amino Acids. 2016;48:2453–65. PubMed

Barcaccia C, Ghedina A, Lucchin M. Current advances in genomics and breeding of leaf Chicory (Cichorium intybus L) Agriculture. 2016;50:2–24.

Saed M, El-Hack MEA, Alagawany M, Arain MA, Arif M, Mirza MA, et al. Chicory (Cichorium intybus) Herb: Chemical Composition, Pharmacology, Nutritional and Healthical Applications. Int J Pharmacol. 2017;13:351–60.

Street RA, Sidana J, Prinsloo G. Cichorium intybus: Traditional uses, phytochemistry, pharmacology, and toxicology. Evid Based Complement Alternat Med. 2013:579319. PubMed PMC

Fernandez-Carmona J, Blas E, Pascual JJ, Maertens L, Gidenne T, Xiccato G, et al. Recommendations and guidelines for applied nutrition experiments in rabbits. World Rabbit Sci. 2005;13:209–28.

De Blas C, Mateos GG, De Blas C, Wiseman J., editors. The Nutrition of the Rabbit. 2nd ed. Wallingford: CABI International; 2010. Feed formulation; pp. 222–32.

Van Soest PJ, Robertson JB, Lewis BA, Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci. 1991;74:3583–97. PubMed

AOAC International. Official Methods of Analysis. 18th ed. USA: Assoc Off Anal Chem Arlington VA; 2005.

AOAC. Official Methods of Analysis. 16th Ed. USA: Assoc Off Anal Chem Arlington VA; 2005.

Freeman HJ. Dietary compliance in celiac disease. World J Gastroenterol. 2017;21:2635–9. PubMed PMC

Aziz I, Dwivedi K, Sanders DS. From coeliac disease to noncoeliac gluten sensitivity; should everyone be gluten free? Curr Opin Gastroenterol. 2016;32:120–7. PubMed

Lerner A, Ramesh A, Matthias T. Are non-celiac autoimmune diseases responsive to gluten-free diet? Int J Celiac Dis. 2017;5:164–7.

Samasca G, Lerner A, Girbovan A, Sur G, Lupan I, Makovicky P, et al. Challenges in gluten-free diet in coeliac disease: Prague consensus. Eur J Clin Invest. 2017;47:394–7. PubMed

Samasca G, Sur G, Lupan I, Deleanu D. Gluten-free diet and quality of life in celiac disease. Gastroenterol Hepatol Bed Bench. 2014;7:139–43. PubMed PMC

Lamacchia C, Camarcia A, Picascia S, Di Luccia A, Gianfranni C. Cereal-based gluten-free food: how to reconcile nutritional and technological properties of wheat proteins with safety for celiac disease patients. Nutrients. 2014;6:575–90. PubMed PMC

Merz M, Kettner L, Langolf E, Appel D, Blank I, Stressler T, et al. Production of wheat gluten hydrolysates with reduced antigenicity employing enzymatic hydrolysis combined with downstream unit operations. J Sci Food Agric. 2016;96:3358–64. PubMed

Drabinska N, Zielinski H, Krupa-Kozak U. Technological benefits of inulin-type fructans application in gluten-free products – A review. Trends Food Sci Technol. 2016;56:149–57.

Drabinska N, Bacyek N, Krupa-Koyak U. Knowledge about coeliac disease: Results of survey conducted among persons screened using a self-administered transglutaminase-based test. Acta Aliment. 2016;46:283–9.

Vymetalkova V, Pardini B, Rosa F, Jiraskova K, Di Gaetano C, Bendova P, et al. Polymorphisms in microRNA binding sites of mucin genes as predictors of clinical outcome in colorectal cancer patients. Carcinogenesis. 2017;38:28–39. PubMed

Santini D, Baldi A, Vincenzi B, Mellone P, Campioni M, Antinori A. Mucin 2 (MUC2) and mucin 5 (MUC5) expression is not associated with prognosis in patients with radically resected ampullary carcinoma. J Clin Pathol. 2007;60:1069–70. PubMed PMC

Castellini C, Cardinali R, Rebollar PG, Dal Bosco A, Jimeno V, Cossu ME. Feeding fresh chicory (Chicoria intybus) to young rabbits: Performance, development of gastro-intestinal tract and immune functions of appendix and Peyer's patch. Anim Feed Sci Technol. 2007;134:56–65.

Lee GJ, Kao JY. Recent advances in pediatric celiac disease. Expert Rev Gastroenterol Hepatol. 2017;11:583–92. PubMed

Schumann M, Siegmund B, Schulyke JD, Fromm M. Celiac disease: Role of the epithelial barrier. Cell Mol Gastroenterol Hepatol. 2017;3:150–62. PubMed PMC

Al-Snafi AE. Medical importance of Cichorium intybus-a review. IOSR J Pharmm. 2016;6:41–56.