Hepatitis E virus in archived sera from wild boars (Sus scrofa), Czech Republic
Language English Country Germany Media print-electronic
Document type Journal Article
Grant support
17-31921A
Ministerstvo Zdravotnictví Ceské Republiky
PubMed
30014614
DOI
10.1111/tbed.12950
Knihovny.cz E-resources
- Keywords
- ELISA, Europe, RT-qPCR, emerging zoonoses, hepatitis E virus, wildlife,
- MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Hepatitis E blood epidemiology veterinary MeSH
- Swine Diseases epidemiology virology MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Swine MeSH
- Sus scrofa virology MeSH
- Hepatitis E virus genetics isolation & purification MeSH
- Disease Reservoirs MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic epidemiology MeSH
To determine whether hepatitis E virus (HEV) was distributed in the population of wild boars in South Moravia between 1990 and 2008, a total of 366 samples of archived sera from wild boars were investigated using serological (commercial ELISA) and molecular (RT-qPCR) methods. A total of 31 (8.5%) wild boars were seropositive, and from two of them, RNA sequences were recovered by nested RT-PCR. The presented results, with one of the oldest animal's HEV-positive serum (collected in 1990), suggest that wild boars may be a reservoir of HEV in the Czech Republic and that this virus has been circulating in studied areas for more than 20 years.
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