Protein expression of the transcription factors DMRT1, TCLF5, and OCT4 in selected germ cell neoplasms of the testis
Language English Country United States Media print-electronic
Document type Comparative Study, Journal Article
PubMed
30067948
DOI
10.1016/j.humpath.2018.07.019
PII: S0046-8177(18)30287-9
Knihovny.cz E-resources
- Keywords
- DMRT1, Mixed germ cell–sex cord stromal tumor, OCT4, Seminoma, Spermatocytic tumor, Testis,
- MeSH
- Biopsy MeSH
- Cell Nucleus chemistry pathology MeSH
- Neoplasms, Germ Cell and Embryonal chemistry pathology MeSH
- Immunohistochemistry MeSH
- Humans MeSH
- Biomarkers, Tumor analysis MeSH
- Octamer Transcription Factor-3 analysis MeSH
- Seminiferous Tubules chemistry pathology MeSH
- Seminoma chemistry pathology MeSH
- Spermatocytes chemistry pathology MeSH
- Spermatogenesis MeSH
- Testicular Neoplasms chemistry pathology MeSH
- Basic Helix-Loop-Helix Transcription Factors analysis MeSH
- Transcription Factors analysis MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- DMRT1 protein MeSH Browser
- Biomarkers, Tumor MeSH
- Octamer Transcription Factor-3 MeSH
- POU5F1 protein, human MeSH Browser
- TCFL5 protein, human MeSH Browser
- Basic Helix-Loop-Helix Transcription Factors MeSH
- Transcription Factors MeSH
In the present study, we investigated protein expression of the transcription factors mammalian doublesex and mab-3 related transcription factor 1 (DMRT1), basic helix-loop-helix transcription factor-like 5 (TCLF5), and octamer-binding transcription factor 4 (OCT4) in normal human spermatogenesis, testicular mixed germ cell-sex cord stromal tumor (MGC-SCST), spermatocytic tumor, and seminoma. In normal human spermatogenesis, DMRT1 is expressed in the nuclei of spermatogonia but not in those of more mature germ cells. By way of contrast, TCLF5 is expressed in the nuclei of some clusters of primary spermatocytes that have entered meiosis 1, in secondary spermatocytes, and in round (early) spermatids in the seminiferous tubules of adults during the reproductive years. OCT4 is expressed in primordial germ cells but not in the seminiferous tubules of the normal adult testis during the reproductive years. DMRT1 is expressed in the germ cells of both testicular MGC-SCST and spermatocytic tumor, whereas TCLF5 is not expressed in either neoplasm. These low-grade neoplasms, however, differ histologically in that all the germ cell nuclei of testicular MGC-SCST resemble spermatogonia, whereas in spermatocytic tumor, the nuclei of the medium-sized and large cells resemble those of primary spermatocytes. Both neoplasms lack expression of OCT4. By way of contrast, in seminoma, a fully malignant testicular germ cell tumor, the germ cell nuclei express OCT4 but do not express either DMRT1 or TCLF5.
Department of Pathology Charles University Faculty of Medicine in Pilsen Pilsen 30460 Czech Republic
Department of Pathology Indiana University School of Medicine Indianapolis IN 46202 USA
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