One of the most studied phosphoinositides is phosphatidylinositol 4,5-bisphosphate (PIP2), which localizes to the plasma membrane, nuclear speckles, small foci in the nucleoplasm, and to the nucleolus in mammalian cells. Here, we show that PIP2 also localizes to the nucleus in prophase I, during the gametogenesis of C. elegans hermaphrodite. The depletion of PIP2 by type I PIP kinase (PPK-1) kinase RNA interference results in an altered chromosome structure and leads to various defects during meiotic progression. We observed a decreased brood size and aneuploidy in progeny, defects in synapsis, and crossover formation. The altered chromosome structure is reflected in the increased transcription activity of a tightly regulated process in prophase I. To elucidate the involvement of PIP2 in the processes during the C. elegans development, we identified the PIP2-binding partners, leucine-rich repeat (LRR-1) protein and proteasome subunit beta 4 (PBS-4), pointing to its involvement in the ubiquitin⁻proteasome pathway.

Department of Biology of the Cell Nucleus Institute of Molecular Genetics of the Czech Academy of Sciences v v i Prague 142 20 Czech Republic

Department of Epigenetics of the Cell Nucleus Institute of Molecular Genetics of the Czech Academy of Sciences v v i Division BIOCEV Vestec 252 50 Czech Republic

Microscopy Centre Institute of Molecular Genetics of the Czech Academy of Sciences v v i Prague 142 20 Czech Republic

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Lamin A/C and PI(4,5)P2-A Novel Complex in the Cell Nucleus

Nuclear Phosphoinositides-Versatile Regulators of Genome Functions