Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I transcription
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23591814
DOI
10.1242/jcs.123661
PII: jcs.123661
Knihovny.cz E-zdroje
- Klíčová slova
- Fibrillarin, Nucleolus, PIP2, Transcription, UBF,
- MeSH
- buněčné jadérko genetika metabolismus MeSH
- chromozomální proteiny, nehistonové genetika metabolismus MeSH
- DNA vazebné proteiny genetika metabolismus MeSH
- fosfatidylinositol-4,5-difosfát genetika metabolismus MeSH
- genetická transkripce genetika MeSH
- HeLa buňky MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- prekurzory RNA genetika metabolismus MeSH
- promotorové oblasti (genetika) genetika MeSH
- RNA-polymerasa I genetika metabolismus MeSH
- transkripční iniciační komplex Pol1 - proteiny genetika metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- chromozomální proteiny, nehistonové MeSH
- DNA vazebné proteiny MeSH
- fibrillarin MeSH Prohlížeč
- fosfatidylinositol-4,5-difosfát MeSH
- prekurzory RNA MeSH
- RNA-polymerasa I MeSH
- transcription factor UBF MeSH Prohlížeč
- transkripční iniciační komplex Pol1 - proteiny MeSH
RNA polymerase I (Pol I) transcription is essential for the cell cycle, growth and protein synthesis in eukaryotes. In the present study, we found that phosphatidylinositol 4,5-bisphosphate (PIP2) is a part of the protein complex on the active ribosomal promoter during transcription. PIP2 makes a complex with Pol I and the Pol I transcription factor UBF in the nucleolus. PIP2 depletion reduces Pol I transcription, which can be rescued by the addition of exogenous PIP2. In addition, PIP2 also binds directly to the pre-rRNA processing factor fibrillarin (Fib), and co-localizes with nascent transcripts in the nucleolus. PIP2 binding to UBF and Fib modulates their binding to DNA and RNA, respectively. In conclusion, PIP2 interacts with a subset of Pol I transcription machinery, and promotes Pol I transcription.
Citace poskytuje Crossref.org
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