Correct species identification (reclassification in CNCTC) of strains of Staphylococcus intermedius-group can improve an insight into their evolutionary history
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
Grantová podpora
MH CZ - DRO ("The National Institute of Public Health - NIPH, 75010330").
MH CZ - DRO
SVV 260398
SVV
CEP - Centrální evidence projektů
PubMed
30238302
DOI
10.1007/s12223-018-0647-7
PII: 10.1007/s12223-018-0647-7
Knihovny.cz E-zdroje
- MeSH
- bakteriální proteiny genetika MeSH
- bakteriologické techniky MeSH
- biologická evoluce * MeSH
- DNA bakterií genetika MeSH
- lidé MeSH
- polymerázová řetězová reakce MeSH
- polymorfismus délky restrikčních fragmentů MeSH
- repetitivní sekvence nukleových kyselin genetika MeSH
- sekvenční analýza DNA MeSH
- stafylokokové infekce mikrobiologie veterinární MeSH
- Staphylococcus intermedius klasifikace genetika izolace a purifikace MeSH
- Staphylococcus klasifikace genetika izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- bakteriální proteiny MeSH
- DNA bakterií MeSH
A group of 59 putative strains of Staphylococcus intermedius/Staphylococcus pseudintermedius deposited in the Czech National Collection of Type Cultures (CNCTC, National Institute for Public Health, Prague, Czech Republic) and the National Reference Laboratory for Staphylococci (NRL for Staphylococci, National Institute for Public Health, Prague, Czech Republic) was reclassified using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). There the biggest human collection of S. pseudintermedius in Europe was analysed; 44 samples (75%) were of human origin. Twenty-two percent (n = 13) of the strains were isolated from animals, and two staphylococci were of unknown origin. This study revealed the prevalence of Staphylococcus pseudintermedius (94%, n = 53) vs. Staphylococcus intermedius (6%, n = 6) in the collection of human and veterinary staphylococci after reclassification. Results of PCR-RFLP analysis were verified by comparison with a repetitive element sequence-based polymerase chain reaction (Rep-PCR) analysis on 26 (44%) randomly selected strains. Due to a low-resolution ability of PCR-RFLP to separate Staphylococcus intermedius from Staphylococcus delphini, four isolates of Staphylococcus intermedius were biochemically verified further to exclude the presence of Staphylococcus delphini in the collection. Our results indicate that S. intermedius and S. pseudintermedius have occurred independently over an age-long period of their co-evolution.
Czech National Collection of Type Cultures National Institute of Public Health Prague Czech Republic
Faculty of Medicine in Hradec Králové Charles University Hradec Králové Czech Republic
Zobrazit více v PubMed
J Clin Microbiol. 1999 Jun;37(6):1661-9 PubMed
Int J Syst Evol Microbiol. 2005 Jul;55(Pt 4):1569-73 PubMed
J Clin Microbiol. 2006 Dec;44(12):4609-12 PubMed
Vet Microbiol. 2007 Mar 31;121(1-2):170-6 PubMed
J Clin Microbiol. 2007 Sep;45(9):2770-8 PubMed
J Bacteriol. 2007 Dec;189(23):8685-92 PubMed
Vet Microbiol. 2009 Jan 1;133(1-2):206-7 PubMed
J Clin Microbiol. 2009 Feb;47(2):469-71 PubMed
J Clin Microbiol. 2010 Mar;48(3):765-9 PubMed
Vet Dermatol. 2009 Oct;20(5-6):490-5 PubMed
J Antimicrob Chemother. 2010 Sep;65(9):2047-8 PubMed
J Vet Diagn Invest. 2010 Sep;22(5):756-9 PubMed
Syst Appl Microbiol. 2010 Dec;33(8):451-6 PubMed
Folia Microbiol (Praha). 2011 Sep;56(5):415-22 PubMed
J Small Anim Pract. 2012 Mar;53(3):147-54 PubMed
Front Cell Infect Microbiol. 2012 Apr 18;2:44 PubMed
Clin Microbiol Infect. 2014 Oct;20(10):1007-15 PubMed
J Clin Microbiol. 2014 Aug;52(8):3118-20 PubMed
BMC Microbiol. 2015 May 21;15:109 PubMed
Open Forum Infect Dis. 2015 Jul 17;2(3):ofv110 PubMed
J Food Sci Technol. 2016 Jan;53(1):42-9 PubMed
Pol J Vet Sci. 2016;19(1):7-14 PubMed
Diagn Microbiol Infect Dis. 2016 Aug;85(4):471-6 PubMed
Vet Microbiol. 2017 Nov;211:103-106 PubMed
Folia Microbiol (Praha). 2018 Nov;63(6):743-747 PubMed
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