Discovery of multi-megabase polymorphic inversions by chromosome conformation capture sequencing in large-genome plant species
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
30256471
DOI
10.1111/tpj.14109
Knihovny.cz E-resources
- Keywords
- Hordeum vulgare, barley, chromosomal inversions, chromosome conformation capture sequencing, genomics, optical mapping, technical advance,
- MeSH
- Chromosome Inversion genetics MeSH
- Chromosomes, Plant genetics MeSH
- Genome, Plant genetics MeSH
- Genotype MeSH
- In Situ Hybridization, Fluorescence MeSH
- Hordeum genetics MeSH
- Chromosome Mapping MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Chromosomal inversions occur in natural populations of many species, and may underlie reproductive isolation and local adaptation. Traditional methods of inversion discovery are labor-intensive and lack sensitivity. Here, we report the use of three-dimensional contact probabilities between genomic loci as assayed by chromosome-conformation capture sequencing (Hi-C) to detect multi-megabase polymorphic inversions in four barley genotypes. Inversions are validated by fluorescence in situ hybridization and Bionano optical mapping. We propose Hi-C as a generally applicable method for inversion discovery in natural populations.
References provided by Crossref.org
Flow Sorting-Assisted Optical Mapping
Large tandem duplications affect gene expression, 3D organization, and plant-pathogen response
