Discovery of multi-megabase polymorphic inversions by chromosome conformation capture sequencing in large-genome plant species
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
30256471
DOI
10.1111/tpj.14109
Knihovny.cz E-zdroje
- Klíčová slova
- Hordeum vulgare, barley, chromosomal inversions, chromosome conformation capture sequencing, genomics, optical mapping, technical advance,
- MeSH
- chromozomální inverze genetika MeSH
- chromozomy rostlin genetika MeSH
- genom rostlinný genetika MeSH
- genotyp MeSH
- hybridizace in situ fluorescenční MeSH
- ječmen (rod) genetika MeSH
- mapování chromozomů MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Chromosomal inversions occur in natural populations of many species, and may underlie reproductive isolation and local adaptation. Traditional methods of inversion discovery are labor-intensive and lack sensitivity. Here, we report the use of three-dimensional contact probabilities between genomic loci as assayed by chromosome-conformation capture sequencing (Hi-C) to detect multi-megabase polymorphic inversions in four barley genotypes. Inversions are validated by fluorescence in situ hybridization and Bionano optical mapping. We propose Hi-C as a generally applicable method for inversion discovery in natural populations.
Citace poskytuje Crossref.org
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