Concurrent infection of monocyte-derived macrophages with porcine reproductive and respiratory syndrome virus and Haemophilus parasuis: A role of IFNα in pathogenesis of co-infections
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články
PubMed
30322536
DOI
10.1016/j.vetmic.2018.09.016
PII: S0378-1135(18)30709-0
Knihovny.cz E-zdroje
- Klíčová slova
- Co-infections, Haemophilus parasuis, IFNα, Macrophages, Porcine reproductive and respiratory syndrome virus,
- MeSH
- bronchoalveolární laváž MeSH
- cytokiny genetika imunologie MeSH
- Haemophilus parasuis imunologie patogenita MeSH
- hemofilové infekce mikrobiologie veterinární MeSH
- interferon alfa imunologie farmakologie MeSH
- makrofágy účinky léků imunologie mikrobiologie virologie MeSH
- nemoci prasat mikrobiologie virologie MeSH
- prasata MeSH
- reprodukční a respirační syndrom prasat virologie MeSH
- viabilita buněk MeSH
- virus reprodukčního a respiračního syndromu prasat imunologie patogenita MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- cytokiny MeSH
- interferon alfa MeSH
Porcine reproductive and respiratory syndrome virus (PRRSV) predisposes pigs to secondary bacterial infection caused by Haemophilus parasuis. The aim of the present study was to analyse the immune response of monocyte-derived macrophages (MDMs), serving as a model of macrophages accumulating at the site of inflammation. The second part of the study was focused on the role of IFNα in the production of inflammatory cytokines in co-infected MDMs. Concurrent infection with PRRSV and H. parasuis decreased gene expression of pro-inflammatory cytokines (IL-1β, IL-8) in MDMs in comparison with MDMs infected with PRRSV or H. parasuis alone. Our data showed that MDMs express IFNα after PRRSV infection. Thereafter, we exposed cells to the experimental addition of IFNα and a subsequent infection with H. parasuis, and detected a decreased expression/production of pro-inflammatory cytokines. Thus, we assume that IFNα, produced after PRRSV infection, could affect the immune response of monocyte-derived macrophages. Down-regulation of pro-inflammatory cytokine expression in inflammatory macrophages may allow the development of secondary bacterial infections in pigs.
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