Importance of Cadherins Methylation in Ovarian Cancer: a Next Generation Sequencing Approach
Language English Country Switzerland Media print-electronic
Document type Journal Article
Grant support
UHHK, 00179906
Ministerstvo Zdravotnictví Ceské Republiky
BBMRI, LM2015089
Ministerstvo Školství, Mládeže a Tělovýchovy
PubMed
30368729
DOI
10.1007/s12253-018-0500-y
PII: 10.1007/s12253-018-0500-y
Knihovny.cz E-resources
- Keywords
- Cadherins, Epigenetics, Methylation, Next generation sequencing, Ovarian cancer,
- MeSH
- Adult MeSH
- Cadherins genetics MeSH
- Middle Aged MeSH
- Humans MeSH
- DNA Methylation * MeSH
- Young Adult MeSH
- Adenocarcinoma, Mucinous genetics pathology MeSH
- Biomarkers, Tumor genetics MeSH
- Endometrial Neoplasms genetics pathology MeSH
- Ovarian Neoplasms genetics pathology MeSH
- Follow-Up Studies MeSH
- Prognosis MeSH
- Promoter Regions, Genetic MeSH
- Gene Expression Regulation, Neoplastic * MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Cystadenocarcinoma, Serous genetics pathology MeSH
- Case-Control Studies MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Young Adult MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- H-cadherin MeSH Browser
- Cadherins MeSH
- Biomarkers, Tumor MeSH
Epigenetic aberrations are well known to play an important role in carcinogenesis, and also have a great potential to serve as biomarkers in many types of cancers, including ovarian cancer in which sensitive and specific biomarkers and detection methods are critically needed. The aim of this study was to investigate methylation of cadherin genes CDH10, CDH13 and CDH18 in ovarian cancer tissue by comparison with control tissue. The study group consisted of 38 patients with ovarian cancer and 25 control patients. For detection of epigenetic events we used next generation sequencing, the most important data were confirmed using high-resolution melting analysis and real-time PCR. We observed significantly higher methylation in CDH13, sporadic methylation in CDH10 and loss of methylation in CDH18 in the ovarian cancer group compared with the control group. These observations suggest that changes in methylation of cadherin genes may be one of the major mechanisms associated with ovarian cancer progression. In addition, because of the high frequency of methylation of the CDH13 gene in the early stages of ovarian cancer, the analyzed CpG sites might be good targets for next study of potential ovarian cancer screening biomarkers.
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