A high-throughput pipeline for validation of antibodies
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
30377371
DOI
10.1038/s41592-018-0179-8
PII: 10.1038/s41592-018-0179-8
Knihovny.cz E-zdroje
- MeSH
- hmotnostní spektrometrie MeSH
- imunoprecipitace MeSH
- lidé MeSH
- nádorové buňky kultivované MeSH
- nádorové proteiny imunologie metabolismus MeSH
- nádory imunologie metabolismus MeSH
- proteomika metody MeSH
- protilátky imunologie MeSH
- rychlé screeningové testy metody normy MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- nádorové proteiny MeSH
- protilátky MeSH
Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we used preparative gel electrophoresis to develop a capture format for WB. Fractions with soluble, size-separated proteins facilitated parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). This pipeline provided the means for large-scale implementation of antibody validation concepts proposed by an international working group on antibody validation (IWGAV).
Department of Immunology University of Oslo and Oslo University Hospital Oslo Norway
Department of Pathology Oslo University Hospital Oslo Norway
K G Jebsen Center for Cancer Immunotherapy University of Oslo Oslo Norway
K G Jebsen Inflammation Research Centre University of Oslo Oslo Norway
NMI Natural and Medical Sciences Institute University of Tübingen Reutlingen Germany
Norwegian Computing Center Oslo Norway
SeekQuence Mountain View CA USA
The Brain Institute Universidade Federal do Rio Grande do Norte Natal Brazil
Citace poskytuje Crossref.org
