Radiolabeling of the antibody IgG M75 for epitope of human carbonic anhydrase IX by 61Cu and 64Cu and its biological testing
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
30391716
DOI
10.1016/j.apradiso.2018.10.021
PII: S0969-8043(18)30330-0
Knihovny.cz E-zdroje
- Klíčová slova
- Carbonic anhydrase IX, Cu-61, Cu-64, PET, Tumour diagnostics, “phosphinate”,
- MeSH
- antigeny nádorové imunologie MeSH
- buňky HT-29 MeSH
- imunoglobulin G chemie MeSH
- imunokonjugáty chemie farmakokinetika MeSH
- karboanhydrasa IX imunologie MeSH
- kolorektální nádory diagnostické zobrazování enzymologie MeSH
- lidé MeSH
- monoklonální protilátky chemie farmakokinetika MeSH
- myši nahé MeSH
- myši MeSH
- pozitronová emisní tomografie MeSH
- radiofarmaka chemie farmakokinetika MeSH
- radioimunodetekce MeSH
- radioizotopy mědi chemie farmakokinetika MeSH
- tkáňová distribuce MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antigeny nádorové MeSH
- CA9 protein, human MeSH Prohlížeč
- Copper-61 MeSH Prohlížeč
- Copper-64 MeSH Prohlížeč
- imunoglobulin G MeSH
- imunokonjugáty MeSH
- karboanhydrasa IX MeSH
- M75 monoclonal antibody MeSH Prohlížeč
- monoklonální protilátky MeSH
- radiofarmaka MeSH
- radioizotopy mědi MeSH
Human carbonic anhydrase IX is a membrane enzyme that is significantly expressed in some types of cancer cells, while copper radioisotopes offer wide range of diagnostic, therapeutic and theranostic properties. The work was focused on a new approach to the labelling of antibody IgG M75 for epitope human carbonic anhydrase IX with copper radioisotopes 61Cu and 64Cu and its in vivo testing in mice with inoculated colorectal cancer. Monoclonal antibody IgG M75 for epitope human carbonic anhydrase IX was successfully conjugated with copper-specific chelator "phosphinate" and labelled with 61Cu and 64Cu The obtained molecule has considerable potential as a radioimmuno pharmaceutical suitable for imaging of tumours expressing carbonic anhydrase IX by positron emission tomography (PET).
Department of Inorganic Chemistry Faculty of Science Charles University Prague Czech Republic
Department of Radiopharmaceuticals Nuclear Physics Institute of the CAS Czech Republic
Laboratory of Structural Biology Institute of Molecular Genetics of the CAS Czech Republic
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