Radiolabeling of the antibody IgG M75 for epitope of human carbonic anhydrase IX by 61Cu and 64Cu and its biological testing
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
30391716
DOI
10.1016/j.apradiso.2018.10.021
PII: S0969-8043(18)30330-0
Knihovny.cz E-resources
- Keywords
- Carbonic anhydrase IX, Cu-61, Cu-64, PET, Tumour diagnostics, “phosphinate”,
- MeSH
- Antigens, Neoplasm immunology MeSH
- HT29 Cells MeSH
- Immunoglobulin G chemistry MeSH
- Immunoconjugates chemistry pharmacokinetics MeSH
- Carbonic Anhydrase IX immunology MeSH
- Colorectal Neoplasms diagnostic imaging enzymology MeSH
- Humans MeSH
- Antibodies, Monoclonal chemistry pharmacokinetics MeSH
- Mice, Nude MeSH
- Mice MeSH
- Positron-Emission Tomography MeSH
- Radiopharmaceuticals chemistry pharmacokinetics MeSH
- Radioimmunodetection MeSH
- Copper Radioisotopes chemistry pharmacokinetics MeSH
- Tissue Distribution MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Antigens, Neoplasm MeSH
- CA9 protein, human MeSH Browser
- Copper-61 MeSH Browser
- Copper-64 MeSH Browser
- Immunoglobulin G MeSH
- Immunoconjugates MeSH
- Carbonic Anhydrase IX MeSH
- M75 monoclonal antibody MeSH Browser
- Antibodies, Monoclonal MeSH
- Radiopharmaceuticals MeSH
- Copper Radioisotopes MeSH
Human carbonic anhydrase IX is a membrane enzyme that is significantly expressed in some types of cancer cells, while copper radioisotopes offer wide range of diagnostic, therapeutic and theranostic properties. The work was focused on a new approach to the labelling of antibody IgG M75 for epitope human carbonic anhydrase IX with copper radioisotopes 61Cu and 64Cu and its in vivo testing in mice with inoculated colorectal cancer. Monoclonal antibody IgG M75 for epitope human carbonic anhydrase IX was successfully conjugated with copper-specific chelator "phosphinate" and labelled with 61Cu and 64Cu The obtained molecule has considerable potential as a radioimmuno pharmaceutical suitable for imaging of tumours expressing carbonic anhydrase IX by positron emission tomography (PET).
Department of Inorganic Chemistry Faculty of Science Charles University Prague Czech Republic
Department of Radiopharmaceuticals Nuclear Physics Institute of the CAS Czech Republic
Laboratory of Structural Biology Institute of Molecular Genetics of the CAS Czech Republic
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