A diversity of amoebae colonise the gills of farmed Atlantic salmon (Salmo salar) with amoebic gill disease (AGD)
Language English Country Germany Media print-electronic
Document type Journal Article
PubMed
30447480
DOI
10.1016/j.ejop.2018.10.003
PII: S0932-4739(18)30087-7
Knihovny.cz E-resources
- Keywords
- AGD, Amoebozoa, Aquaculture, Atlantic salmon, Discosea, Tubulinea,
- MeSH
- Amebiasis parasitology veterinary MeSH
- Amoebozoa classification genetics ultrastructure MeSH
- Biodiversity * MeSH
- Phylogeny MeSH
- Microscopy MeSH
- Fish Diseases parasitology MeSH
- Electron Transport Complex IV genetics MeSH
- RNA, Ribosomal, 18S genetics MeSH
- Salmo salar parasitology MeSH
- Microscopy, Electron, Transmission MeSH
- Gills parasitology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Electron Transport Complex IV MeSH
- RNA, Ribosomal, 18S MeSH
Neoparamoeba perurans is the aetiological agent of amoebic gill disease (AGD) in salmonids, however multiple other amoeba species colonise the gills and their role in AGD is unknown. Taxonomic assessments of these accompanying amoebae on AGD-affected salmon have previously been based on gross morphology alone. The aim of the present study was to document the diversity of amoebae colonising the gills of AGD-affected farmed Atlantic salmon using a combination of morphological and sequence-based taxonomic methods. Amoebae were characterised morphologically via light microscopy and transmission electron microscopy, and by phylogenetic analyses based on the 18S rRNA gene and cytochrome oxidase subunit I (COI) gene. In addition to N. perurans, 11 other amoebozoans were isolated from the gills, and were classified within the genera Neoparamoeba, Paramoeba, Vexillifera, Pseudoparamoeba, Vannella and Nolandella. In some cases, such as Paramoeba eilhardi, this is the first time this species has been isolated from the gills of teleost fish. Furthermore, sequencing of both the 18S rRNA and COI gene revealed significant genetic variation within genera. We highlight that there is a far greater diversity of amoebae colonising AGD-affected gills than previously established.
Faculty of Science Masaryk University Kotlářská 2 611 37 Brno Czech Republic
The University of Queensland School of Biological Sciences Brisbane Queensland 4072 Australia
References provided by Crossref.org
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