The choice of embedding media affects image quality, tissue R2* , and susceptibility behaviors in post-mortem brain MR microscopy at 7.0T
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
15-25602A
Czech Ministry of Health - International
Q27/LF1
Charles University - International
Q28/LF1;
Charles University - International
CZ.2.16/3.1.00/24509
OPPK - International
1210251
Helmholtz Alliance ICEMED - International
203147/Z/16/Z
Wellcome - International
PubMed
30506939
DOI
10.1002/mrm.27595
Knihovny.cz E-zdroje
- Klíčová slova
- ex vivo imaging, formaldehyde fixation, post-mortem imaging, quantitative susceptibility mapping, ultra-high field MRI,
- MeSH
- ethery MeSH
- fluorokarbony MeSH
- formaldehyd MeSH
- fosfáty MeSH
- kontrastní látky MeSH
- lidé středního věku MeSH
- lidé MeSH
- magnetická rezonanční tomografie přístrojové vybavení metody MeSH
- mapování mozku metody MeSH
- mozek diagnostické zobrazování patologie MeSH
- odběr biologického vzorku MeSH
- oxid deuteria MeSH
- pitva přístrojové vybavení metody MeSH
- počítačové zpracování obrazu MeSH
- poměr signál - šum MeSH
- sefarosa chemie MeSH
- senioři MeSH
- zalévání tkání přístrojové vybavení MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ethery MeSH
- fluorokarbony MeSH
- formaldehyd MeSH
- fosfáty MeSH
- kontrastní látky MeSH
- oxid deuteria MeSH
- perfluoropolyether MeSH Prohlížeč
- sefarosa MeSH
PURPOSE: The quality and precision of post-mortem MRI microscopy may vary depending on the embedding medium used. To investigate this, our study evaluated the impact of 5 widely used media on: (1) image quality, (2) contrast of high spatial resolution gradient-echo (T1 and T2* -weighted) MR images, (3) effective transverse relaxation rate (R2* ), and (4) quantitative susceptibility measurements (QSM) of post-mortem brain specimens. METHODS: Five formaldehyde-fixed brain slices were scanned using 7.0T MRI in: (1) formaldehyde solution (formalin), (2) phosphate-buffered saline (PBS), (3) deuterium oxide (D2 O), (4) perfluoropolyether (Galden), and (5) agarose gel. SNR and contrast-to-noise ratii (SNR/CNR) were calculated for cortex/white matter (WM) and basal ganglia/WM regions. In addition, median R2* and QSM values were extracted from caudate nucleus, putamen, globus pallidus, WM, and cortical regions. RESULTS: PBS, Galden, and agarose returned higher SNR/CNR compared to formalin and D2 O. Formalin fixation, and its use as embedding medium for scanning, increased tissue R2* . Imaging with agarose, D2 O, and Galden returned lower R2* values than PBS (and formalin). No major QSM offsets were observed, although spatial variance was increased (with respect to R2* behaviors) for formalin and agarose. CONCLUSIONS: Embedding media affect gradient-echo image quality, R2* , and QSM in differing ways. In this study, PBS embedding was identified as the most stable experimental setup, although by a small margin. Agarose and Galden were preferred to formalin or D2 O embedding. Formalin significantly increased R2* causing noisier data and increased QSM variance.
Berlin Ultrahigh Field Facility Berlin Germany
Department of Biomedical Engineering University Basel Switzerland
Department of Pathology and Molecular Medicine Thomayer Hospital Praha Czech Republic
Experimental and Clinical Research Center Berlin Germany
German Center for Neurodegenerative Diseases Magdeburg Germany
Institute of Neuropathology University Medicine Göttingen Göttingen Germany
Medical Imaging Analysis Center AG Basel Switzerland
NeuroCure Clinical Research Center Charité Universitaetsmedizin Berlin Germany
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