Nuclear Genome Size in Contrast to Sex Chromosome Number Variability in the Human Bed Bug, Cimex lectularius (Heteroptera: Cimicidae)
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
30729668
DOI
10.1002/cyto.a.23729
Knihovny.cz E-zdroje
- Klíčová slova
- Cimex lectularius, chromosome number variability, cytogenetics, flow cytometry, genome size, holokinetic chromosomes, internal standard,
- MeSH
- barvení a značení MeSH
- buněčné jádro chemie genetika MeSH
- cytogenetika MeSH
- délka genomu * MeSH
- indoly MeSH
- meióza genetika MeSH
- metafáze genetika MeSH
- mitóza genetika MeSH
- pohlavní chromozomy * MeSH
- propidium MeSH
- průtoková cytometrie MeSH
- štěnice genetika MeSH
- zastoupení bazí MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DAPI MeSH Prohlížeč
- indoly MeSH
- propidium MeSH
The human bed bug Cimex lectularius is one of the most prevalent human ectoparasites in temperate climate zones. The cytogenetic features of this resilient pest include holokinetic chromosomes, special chromosome behavior in meiosis, and numerical variation of chromosomes, where the diploid number ranges from 26 + X1 X2 Y to 26 + X1-20 Y. It is desirable to assess the nuclear DNA content of various cytotypes for a further detailed study of the C. lectularius genome. Detailed knowledge of the DNA content of this parasite could also clarify the origin of additional chromosomes. The average nuclear genome size C. lectularius with 2n = 26 + X1 X2 Y is 2C = 1.94 pg for males and 1.95 pg for females. There is a significant correlation between genome size and the number of chromosomes, but in some specimens with additional chromosomes, nuclear genome size decreases or remains average. Several species used as the internal reference standard were tested for further investigations of genome size in C. lectularius, and the plant Solanum pseudocaspicum turned out to be the most suitable. © 2019 International Society for Advancement of Cytometry.
Faculty of Science Department of Botany Charles University Praha Czech Republic
Faculty of Science Department of Zoology Charles University Praha Czech Republic
Institute of Entomology Biological Centre Czech Academy of Science České Budějovice Czech Republic
Citace poskytuje Crossref.org
