The plant Pontin and Reptin homologues, RuvBL1 and RuvBL2a, colocalize with TERT and TRB proteins in vivo, and participate in telomerase biogenesis
Jazyk angličtina Země Velká Británie, Anglie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
30834599
DOI
10.1111/tpj.14306
Knihovny.cz E-zdroje
- Klíčová slova
- Arabidopsis, AtPOT1a, AtRuvBL, AtTERT, AtTRB, Pontin, Reptin, nucleolus, telomerase assembly,
- MeSH
- Arabidopsis genetika metabolismus MeSH
- buněčné jadérko metabolismus MeSH
- DNA-helikasy metabolismus MeSH
- fylogeneze MeSH
- jaderné proteiny MeSH
- katalytická doména MeSH
- proteiny huseníčku genetika metabolismus MeSH
- proteiny vázající RNA metabolismus MeSH
- proteiny vázající telomery metabolismus MeSH
- telomerasa metabolismus MeSH
- telomery metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA-helikasy MeSH
- jaderné proteiny MeSH
- NAP57 protein, Arabidopsis MeSH Prohlížeč
- POT1A protein, Arabidopsis MeSH Prohlížeč
- proteiny huseníčku MeSH
- proteiny vázající RNA MeSH
- proteiny vázající telomery MeSH
- telomerasa MeSH
- TERT protein, Arabidopsis MeSH Prohlížeč
- TRB3 protein, Arabidopsis MeSH Prohlížeč
Telomerase maturation and recruitment to telomeres is regulated by several telomerase- and telomere-associated proteins. Among a number of proteins, human Pontin and Reptin play critical roles in telomerase biogenesis. Here we characterized plant orthologues of Pontin and Reptin, RuvBL1 and RuvBL2a, respectively, and show association of Arabidopsis thaliana RuvBL1 (AtRuvBL1) with the catalytic subunit of telomerase (AtTERT) in the nucleolus in vivo. In contrast to mammals, interactions between AtTERT and AtRuvBL proteins in A. thaliana are not direct and they are rather mediated by one of the Arabidopsis thaliana Telomere Repeat Binding (AtTRB) proteins. We further show that plant orthologue of dyskerin, named AtCBF5, is indirectly associated with AtTRB proteins but not with the AtRuvBL proteins in the plant nucleus/nucleolus, and interacts with the Protection of telomere 1 (AtPOT1a) in the nucleolus or cytoplasmic foci. Our genome-wide phylogenetic analyses identify orthologues in RuvBL protein family within the plant kingdom. Dysfunction of AtRuvBL genes in heterozygous T-DNA insertion A. thaliana mutants results in reduced telomerase activity and indicate the involvement of AtRuvBL in plant telomerase biogenesis.
Citace poskytuje Crossref.org
Telomerase Interaction Partners-Insight from Plants
Composition and Function of Telomerase-A Polymerase Associated with the Origin of Eukaryotes
Tidying-up the plant nuclear space: domains, functions, and dynamics
Telomerase RNAs in land plants
