Production of pyruvic acid from glycerol by Yarrowia lipolytica
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
PubMed
30888634
DOI
10.1007/s12223-019-00695-2
PII: 10.1007/s12223-019-00695-2
Knihovny.cz E-zdroje
- MeSH
- bioreaktory MeSH
- glycerol analýza metabolismus MeSH
- kultivační média chemie MeSH
- kyselina pyrohroznová analýza metabolismus MeSH
- kyseliny ketoglutarové analýza metabolismus MeSH
- techniky vsádkové kultivace MeSH
- thiamin analýza MeSH
- Yarrowia růst a vývoj metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- glycerol MeSH
- kultivační média MeSH
- kyselina pyrohroznová MeSH
- kyseliny ketoglutarové MeSH
- thiamin MeSH
The aim of the study was to screen Yarrowia lipolytica strains for keto acid production and determine optimal conditions for pyruvic acid biosynthesis from glycerol by the best producer. The analyzed parameters were thiamine concentration, medium pH, stirring speed, and substrate concentration. The screening was performed in flask cultures, whereas pyruvic acid production was carried out in 5-L stirred-tank reactor with 2 L of working volume. In total, 24 Y. lipolytica strains were compared for their abilities to produce pyruvic and α-ketoglutaric acids. The total concentration of both acids ranged from 0.1 to 15.03 g/L. Ten strains were selected for keto acid biosynthesis in bioreactor. The Y. lipolytica SKO 6 strain was identified as the best producer of pyruvic acid. In the selected conditions (thiamine concentration 1.5 μg/L, pH 4.0, stirring speed 800 rpm, 150 g/L of glycerol), the strain Y. lipolytica SKO 6 produced 99.3 g/L of pyruvic acid, with process yield of 0.63 g/g and volumetric production rate of 1.18 g/L/h. Higher titer of pyruvic acid was obtained during fed-batch culture with 200 g/L of glycerol, reaching 125.8 g/L from pure glycerol (yield 0.68 g/g) and 124.4 g/L from crude glycerol (yield 0.62 g/g). Results obtained for the strain Y. lipolytica SKO 6 proved the suitability of microbial production of pyruvic acid at industrial scale.
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Bioresour Technol. 2013 Jun;137:124-31 PubMed
Brain Res. 2007 Feb 9;1132(1):1-9 PubMed
Front Microbiol. 2017 Jan 24;8:49 PubMed
World J Microbiol Biotechnol. 2010 Jul;26(7):1217-24 PubMed
Biotechnol Bioeng. 2007 Jul 1;97(4):825-32 PubMed
Appl Microbiol Biotechnol. 2013 Jun;97(12):5517-25 PubMed
Biotechnol Adv. 2011 Nov-Dec;29(6):632-44 PubMed
Lett Appl Microbiol. 2010 Sep;51(3):264-71 PubMed
J Biotechnol. 2007 May 1;129(3):453-60 PubMed
Biotechnol Lett. 2009 Mar;31(3):377-80 PubMed
FEMS Yeast Res. 2014 Sep;14(6):966-76 PubMed
Prog Lipid Res. 2009 Nov;48(6):375-87 PubMed
FEMS Yeast Res. 2007 Jan;7(1):33-9 PubMed
Biotechnol Prog. 2014 Jan-Feb;30(1):19-27 PubMed
J Zhejiang Univ Sci B. 2007 Feb;8(2):98-104 PubMed
Lett Appl Microbiol. 2002;35(4):338-42 PubMed
Nat Biotechnol. 2000 Nov;18(11):1121-2 PubMed
Bioresour Technol. 2011 Sep;102(17):8117-22 PubMed
Nat Commun. 2014;5:3131 PubMed
Lett Appl Microbiol. 2004;39(2):199-206 PubMed
Appl Microbiol Biotechnol. 2001 Nov;57(4):451-9 PubMed
J Biosci Bioeng. 2008 Mar;105(3):169-75 PubMed
Appl Microbiol Biotechnol. 2001 Jun;55(6):680-5 PubMed
J Ind Microbiol Biotechnol. 2012 Sep;39(9):1333-43 PubMed
Appl Microbiol Biotechnol. 2011 Mar;89(5):1519-26 PubMed
Appl Microbiol Biotechnol. 2016 Sep;100(17):7689-97 PubMed
FEMS Yeast Res. 2005 Apr;5(6-7):527-43 PubMed
Chem Biol. 2011 Oct 28;18(10):1201-2 PubMed
Dermatol Surg. 2006 Apr;32(4):526-31 PubMed
Appl Environ Microbiol. 2004 Jan;70(1):159-66 PubMed
Appl Microbiol Biotechnol. 2007 Dec;77(4):751-62 PubMed
J Ind Microbiol Biotechnol. 2010 May;37(5):431-5 PubMed
Bioresour Technol. 2018 Oct;266:125-133 PubMed
