Identification of Narciclasine as an in Vitro Anti-Inflammatory Component of Cyrtanthus contractus by Correlation-Based Metabolomics
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Amaryllidaceae Alkaloids chemistry pharmacology MeSH
- Amaryllidaceae chemistry MeSH
- Anti-Inflammatory Agents, Non-Steroidal chemistry pharmacology MeSH
- Cell Adhesion drug effects MeSH
- Cell Line MeSH
- Human Umbilical Vein Endothelial Cells drug effects MeSH
- Phenanthridines chemistry pharmacology MeSH
- Mass Spectrometry MeSH
- Plant Roots chemistry MeSH
- Humans MeSH
- Metabolomics * MeSH
- Intercellular Adhesion Molecule-1 drug effects metabolism MeSH
- Plant Extracts chemistry pharmacology MeSH
- Cell Survival drug effects MeSH
- Chromatography, High Pressure Liquid MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amaryllidaceae Alkaloids MeSH
- Anti-Inflammatory Agents, Non-Steroidal MeSH
- Phenanthridines MeSH
- Intercellular Adhesion Molecule-1 MeSH
- narciclasine MeSH Browser
- Plant Extracts MeSH
In this study, an extract from the bulbs of Cyrtanthus contractus showed strong anti-inflammatory activity in vitro. The extract was partially separated into 14 fractions and analyzed by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry metabolomics, and the correlation coefficients were calculated between biological activities and metabolite levels. As a result, the top-scoring metabolite narciclasine (1) is proposed as the active principle of C. contractus. This was confirmed by comparing the biological effect of crude extract with that of an authentic standard.
References provided by Crossref.org
Sulfonation of IAA in Urtica eliminates its DR5 auxin activity
