Polar auxin transport plays a pivotal role in plant growth and development. PIN-FORMED (PIN) auxin efflux carriers regulate directional auxin movement by establishing local auxin maxima, minima, and gradients that drive multiple developmental processes and responses to environmental signals. Auxin has been proposed to modulate its own transport by regulating subcellular PIN trafficking via processes such as clathrin-mediated PIN endocytosis and constitutive recycling. Here, we further investigated the mechanisms by which auxin affects PIN trafficking by screening auxin analogs and identified pinstatic acid (PISA) as a positive modulator of polar auxin transport in Arabidopsis (Arabidopsis thaliana). PISA had an auxin-like effect on hypocotyl elongation and adventitious root formation via positive regulation of auxin transport. PISA did not activate SCFTIR1/AFB signaling and yet induced PIN accumulation at the cell surface by inhibiting PIN internalization from the plasma membrane. This work demonstrates PISA to be a promising chemical tool to dissect the regulatory mechanisms behind subcellular PIN trafficking and auxin transport.

Centre for Plant Sciences Faculty of Biological Sciences University of Leeds Leeds LS2 9JT UK

Department of Biochemistry Okayama University of Science Okayama 700 0005 Japan

Graduate School of Science Nagoya University Chikusa Nagoya 464 8602 Japan

Institute of Global Innovation Research Tokyo University of Agriculture and Technology Fuchu shi Tokyo 183 8509 Japan

Institute of Science and Technology Austria 3400 Klosterneuburg Austria

Institute of Transformative Bio Molecules Nagoya University Chikusa Nagoya 464 8601 Japan

Laboratory of Growth Regulators The Czech Academy of Sciences Institute of Experimental Botany and Palacký University CZ 78371 Olomouc Czech Republic

RIKEN Center for Sustainable Resource Science Yokohama Kanagawa 230 0045 Japan

School of Life Sciences University of Warwick Coventry CV4 7AL United Kingdom

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Plant Physiol. 2019 Jun;180(2):708. doi: 10.1104/pp.19.00464. PubMed

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