Auxin is unique among plant hormones due to its directional transport that is mediated by the polarly distributed PIN auxin transporters at the plasma membrane. The canalization hypothesis proposes that the auxin feedback on its polar flow is a crucial, plant-specific mechanism mediating multiple self-organizing developmental processes. Here, we used the auxin effect on the PIN polar localization in Arabidopsis thaliana roots as a proxy for the auxin feedback on the PIN polarity during canalization. We performed microarray experiments to find regulators of this process that act downstream of auxin. We identified genes that were transcriptionally regulated by auxin in an AXR3/IAA17- and ARF7/ARF19-dependent manner. Besides the known components of the PIN polarity, such as PID and PIP5K kinases, a number of potential new regulators were detected, among which the WRKY23 transcription factor, which was characterized in more detail. Gain- and loss-of-function mutants confirmed a role for WRKY23 in mediating the auxin effect on the PIN polarity. Accordingly, processes requiring auxin-mediated PIN polarity rearrangements, such as vascular tissue development during leaf venation, showed a higher WRKY23 expression and required the WRKY23 activity. Our results provide initial insights into the auxin transcriptional network acting upstream of PIN polarization and, potentially, canalization-mediated plant development.

Department of Molecular Biology Max Planck Institute for Developmental Biology Tübingen Germany

Department of Plant Biotechnology and Bioinformatics Ghent University and Center for Plant Systems Biology VIB Ghent Belgium

Department of Plant Physiology Umeå Plant Science Centre Umeå University Umeå Sweden

Department of Plant Physiology University of Potsdam Potsdam Germany

Facultad de Recursos Naturales Renovables Universidad Arturo Prat Iquique Chile

Institute of Science and Technology Klosterneuburg Austria

Laboratory of Growth Regulators Palacký University Olomouc Czech Republic

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