Spontaneously arising channels that transport the phytohormone auxin provide positional cues for self-organizing aspects of plant development such as flexible vasculature regeneration or its patterning during leaf venation. The auxin canalization hypothesis proposes a feedback between auxin signaling and transport as the underlying mechanism, but molecular players await discovery. We identified part of the machinery that routes auxin transport. The auxin-regulated receptor CAMEL (Canalization-related Auxin-regulated Malectin-type RLK) together with CANAR (Canalization-related Receptor-like kinase) interact with and phosphorylate PIN auxin transporters. camel and canar mutants are impaired in PIN1 subcellular trafficking and auxin-mediated PIN polarization, which macroscopically manifests as defects in leaf venation and vasculature regeneration after wounding. The CAMEL-CANAR receptor complex is part of the auxin feedback that coordinates polarization of individual cells during auxin canalization.

Department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences Vienna Austria

Department of Plant Biotechnology and Bioinformatics Ghent University 9052 Ghent Belgium

Faculty of Natural Sciences Institute of Biology Biotechnology and Environmental Protection University of Silesia in Katowice Jagiellońska 28 40 032 Katowice Poland

Gregor Mendel Institute Vienna Austria

Institute of Science and Technology 3400 Klosterneuburg Austria

Laboratory of Biochemistry Wageningen University Stippeneng 4 6708 Wageningen the Netherlands

Laboratory of Growth Regulators The Czech Academy of Sciences Institute of Experimental Botany and Palacký University Šlechtitelů 27 CZ 78371 Olomouc Czech Republic

Mendel Centre for Plant Genomics and Proteomics Central European Institute of Technology Masaryk University Brno Czech Republic

VIB Center for Plant Systems Biology 9052 Ghent Belgium

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ABP1-TMK auxin perception for global phosphorylation and auxin canalization

The Hydrophilic Loop of Arabidopsis PIN1 Auxin Efflux Carrier Harbors Hallmarks of an Intrinsically Disordered Protein

AGC kinases and MAB4/MEL proteins maintain PIN polarity by limiting lateral diffusion in plant cells