Polar subcellular localization of the PIN exporters of the phytohormone auxin is a key determinant of directional, intercellular auxin transport and thus a central topic of both plant cell and developmental biology. Arabidopsis mutants lacking PID, a kinase that phosphorylates PINs, or the MAB4/MEL proteins of unknown molecular function display PIN polarity defects and phenocopy pin mutants, but mechanistic insights into how these factors convey PIN polarity are missing. Here, by combining protein biochemistry with quantitative live-cell imaging, we demonstrate that PINs, MAB4/MELs, and AGC kinases interact in the same complex at the plasma membrane. MAB4/MELs are recruited to the plasma membrane by the PINs and in concert with the AGC kinases maintain PIN polarity through limiting lateral diffusion-based escape of PINs from the polar domain. The PIN-MAB4/MEL-PID protein complex has self-reinforcing properties thanks to positive feedback between AGC kinase-mediated PIN phosphorylation and MAB4/MEL recruitment. We thus uncover the molecular mechanism by which AGC kinases and MAB4/MEL proteins regulate PIN localization and plant development.

Department of Biological Sciences Faculty of Science Hokkaido University Sapporo Hokkaido 060 0810 Japan

Department of Plant Biotechnology and Bioinformatics Ghent University 9052 Ghent Belgium; VIB Center for Plant Systems Biology 9052 Ghent Belgium

Institute of Science and Technology Austria 1190 Vienna Austria

Institute of Science and Technology Austria 3400 Klosterneuburg Austria

Institute of Science and Technology Austria 3400 Klosterneuburg Austria; Department of Experimental Plant Biology Faculty of Science Charles University 12844 Prague Czechia

Institute of Science and Technology Austria 3400 Klosterneuburg Austria; Department of Experimental Plant Biology Faculty of Science Charles University 12844 Prague Czechia; Department of Plant Biotechnology and Bioinformatics Ghent University 9052 Ghent Belgium; VIB Center for Plant Systems Biology 9052 Ghent Belgium

Plant Developmental Genetics Institute of Biology Leiden Leiden University 2333 BE Leiden the Netherlands

Plant Developmental Genetics Institute of Biology Leiden Leiden University 2333 BE Leiden the Netherlands; Plant Ecophysiology Institute of Environmental Biology Utrecht University 3584 CH Utrecht the Netherlands

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Curr Biol. 2021 May 10;31(9):R449-R451. doi: 10.1016/j.cub.2021.03.070. PubMed

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