Microbiological diagnostics of bloodstream infections in Europe-an ESGBIES survey
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
30980927
DOI
10.1016/j.cmi.2019.03.024
PII: S1198-743X(19)30143-0
Knihovny.cz E-resources
- Keywords
- Antimicrobial susceptibility testing, Blood culture, Bloodstream infection, Identification, Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, Microbiological diagnostics, Molecular methods, Preanalytics, Rapid diagnostics, Transport,
- MeSH
- Molecular Diagnostic Techniques methods MeSH
- Diagnostic Tests, Routine methods MeSH
- Humans MeSH
- Microbiological Techniques methods MeSH
- Cross-Sectional Studies MeSH
- Sepsis diagnosis MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Europe MeSH
OBJECTIVES: High-quality diagnosis of bloodstream infections (BSI) is important for successful patient management. As knowledge on current practices of microbiological BSI diagnostics is limited, this project aimed to assess its current state in European microbiological laboratories. METHODS: We performed an online questionnaire-based cross-sectional survey comprising 34 questions on practices of microbiological BSI diagnostics. The ESCMID Study Group for Bloodstream Infections, Endocarditis and Sepsis (ESGBIES) was the primary platform to engage national coordinators who recruited laboratories within their countries. RESULTS: Responses were received from 209 laboratories in 25 European countries. Although 32.5% (68/209) of laboratories only used the classical processing of positive blood cultures (BC), two-thirds applied rapid technologies. Of laboratories that provided data, 42.2% (78/185) were able to start incubating BC in automated BC incubators around-the-clock, and only 13% (25/192) had established a 24-h service to start immediate processing of positive BC. Only 4.7% (9/190) of laboratories validated and transmitted the results of identification and antimicrobial susceptibility testing (AST) of BC pathogens to clinicians 24 h/day. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry from briefly incubated sub-cultures on solid media was the most commonly used approach to rapid pathogen identification from positive BC, and direct disc diffusion was the most common rapid AST method from positive BC. CONCLUSIONS: Laboratories have started to implement novel technologies for rapid identification and AST for positive BC. However, progress is severely compromised by limited operating hours such that current practice of BC diagnostics in Europe complies only partly with the requirements for optimal BSI management.
Clinical Microbiology Laboratory University Hospital Centre 'Mother Theresa' Tirana Albania
Department of Clinical Microbiology Aalborg University Hospital Aalborg Denmark
Department of Clinical Microbiology and Infectious Diseases Hadassah Medical Centre Jerusalem Israel
Department of Infection Control and Epidemiology University Hospitals Leuven Leuven Belgium
Department of Medical Microbiology Faculty of Medicine Medical University of Sofia Sofia Bulgaria
Department of Microbiology University Hospitals Plymouth NHS Trust Plymouth UK
Division of Epidemiology Tel Aviv Sourasky Medical Centre Tel Aviv Israel
Institute of Medical Microbiology University Hospital Münster Münster Germany
Microbiology Laboratory Diagnostic Clinic East Tallinn Central Hospital Tallinn Estonia
National Medicines Institute Warsaw Poland
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