The absence of high-affinity potassium uptake in Candida glabrata, the consequence of the deletion of the TRK1 gene encoding the sole potassium-specific transporter, has a pleiotropic effect. Here, we show that in addition to changes in basic physiological parameters (e.g., membrane potential and intracellular pH) and decreased tolerance to various cell stresses, the loss of high affinity potassium uptake also alters cell-surface properties, such as an increased hydrophobicity and adherence capacity. The loss of an efficient potassium uptake system results in diminished virulence as assessed by two insect host models, Drosophila melanogaster and Galleria mellonella, and experiments with macrophages. Macrophages kill trk1Δ cells more effectively than wild type cells. Consistently, macrophages accrue less damage when co-cultured with trk1Δ mutant cells compared to wild-type cells. We further show that low levels of potassium in the environment increase the adherence of C. glabrata cells to polystyrene and the propensity of C. glabrata cells to form biofilms.

Department of Membrane Transport Institute of Physiology of the Czech Academy of Sciences 14220 Prague 4 Czech Republic

Department of Microbiology and Parasitology Faculty of Pharmacy Complutense University of Madrid and IRYCIS Madrid Spain

Department of Microbiology University of Szeged Interdisciplinary Excellence Centre Szeged Hungary

Department of Molecular Biosciences The Wenner Gren Institute Stockholm University SE 10691 Stockholm Sweden

MTA SZTE Lendület Mycobiome Research Group University of Szeged Szeged Hungary

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Yeast Trk1 Potassium Transporter Gradually Changes Its Affinity in Response to Both External and Internal Signals