Nano-formulated curcumin (Lipodisq™) modulates the local inflammatory response, reduces glial scar and preserves the white matter after spinal cord injury in rats
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
31108112
DOI
10.1016/j.neuropharm.2019.05.018
PII: S0028-3908(19)30167-4
Knihovny.cz E-zdroje
- Klíčová slova
- Astrogliosis, Axonal growth, Inflammatory response, Nanocurcumin, Neuroregeneration, Spinal cord injury,
- MeSH
- bílá hmota účinky léků metabolismus patologie MeSH
- jizva farmakoterapie metabolismus patologie MeSH
- krysa rodu Rattus MeSH
- kurkumin aplikace a dávkování MeSH
- mediátory zánětu antagonisté a inhibitory metabolismus MeSH
- nanočástice aplikace a dávkování MeSH
- neuroglie účinky léků metabolismus patologie MeSH
- poranění míchy farmakoterapie metabolismus patologie MeSH
- potkani Wistar MeSH
- příprava léků MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- kurkumin MeSH
- mediátory zánětu MeSH
A highly water soluble, nano-formulated curcumin was used for the treatment of the experimental model of spinal cord injury (SCI) in rats. Nanocurcumin and a vehicle nanocarrier as a control, were delivered both locally, immediately after the spinal cord injury, and intraperitoneally during the 4 consecutive weeks after SCI. The efficacy of the treatment was assessed using behavioral tests, which were performed during the experiment, weekly for 9 weeks. The behavioral tests (BBB, flat beam test, rotarod, motoRater) revealed a significant improvement in the nanocurcumin treated group, compared to the nanocarrier control. An immunohistochemical analysis of the spinal cord tissue was performed at the end of the experiment and this proved a significant preservation of the white matter tissue, a reduced area of glial scaring and a higher amount of newly sprouted axons in the nanocurcumin treated group. The expression of endogenous genes (Sort1, Fgf2, Irf5, Mrc1, Olig2, Casp3, Gap43, Gfap, Vegf, Nfkβ) and interleukins (IL-1β, TNF-α, IL-6, IL-12, CCL-5, IL-11, IL-10, IL-13) was evaluated by qPCR and showed changes in the expression of the inflammatory cytokines in the first two weeks after SCI.
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