Direct comparison of chitinolytic properties and determination of combinatory effects of mouse chitotriosidase and acidic mammalian chitinase
Language English Country Netherlands Media print-electronic
Document type Journal Article
PubMed
31108147
DOI
10.1016/j.ijbiomac.2019.05.097
PII: S0141-8130(19)31800-8
Knihovny.cz E-resources
- Keywords
- Acidic mammalian chitinase, Chitin substrates, Chitotriosidase, Direct comparison, Mutual effects, Serratia marcescens chitinase B,
- MeSH
- Bacterial Proteins chemistry genetics MeSH
- Chitin chemistry MeSH
- Chitinases chemistry genetics MeSH
- Hexosaminidases chemistry MeSH
- Hydrolysis MeSH
- Colorimetry MeSH
- Hydrogen-Ion Concentration MeSH
- Molecular Weight MeSH
- Mice MeSH
- Recombinant Proteins MeSH
- Substrate Specificity MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Bacterial Proteins MeSH
- Chitin MeSH
- chitinase B, Serratia marcescens MeSH Browser
- Chitinases MeSH
- chitotriosidase MeSH Browser
- Hexosaminidases MeSH
- Recombinant Proteins MeSH
Chitotriosidase (Chit1) and acidic mammalian chitinase (AMCase) have been implicated in food processing and various pathophysiological conditions such as chronic inflammatory diseases. By combination of the colorimetric analysis and fluorophore-assisted carbohydrate electrophoresis (FACE) method, we directly compared the chitinolytic properties of mouse Chit1 and AMCase and determined their combinatory effects in artificial and natural chitin substrates processing. Chit1 and AMCase display different dynamics of chitinolytic properties through acidic to neutral conditions. At pH2.0, the activity of AMCase was higher than that of Chit1 and stronger or comparable with that of Serratia marcescens chitinase B, a well-characterized bacterium chitinase. Changes of degradation products using different substrates indicate that AMCase and Chit1 have diverse properties under various pH conditions. Exposure of the chitin substrates to both Chit1 and AMCase did not indicate any mutual interference of these enzymes and showed no synergistic effect, in contrast to observations regarding some bacterial chitinases. Our results suggest that Chit1 and AMCase have no synergistic effect under physiological conditions.
References provided by Crossref.org
