The effect of hempseed in the diet of laying hens was evaluated at 0, 30, 60 and 90 g/kg concentrations. The aim of the study was to determine the effect of dietary treatment on the performance of hens, the physical characteristics of egg quality, the concentrations of α- and γ-tocopherol and the carotenoid and cholesterol contents of egg yolks, together with the breaking strength of tibial measurements. In light of the obtained results, our study aimed to address the optimal dietary level of hempseed in laying hen diets. Lohmann Brown hens (n = 240) were divided into 4 dietary treatment groups (6 cages per treatment) with 10 hens per cage. The experiment lasted for 12 weeks. The level of nutrients in all diets (wheat-corn) was well balanced. The dietary metabolisable energy was adjusted using rapeseed oil. The addition of 30 g/kg of hempseed to the diet significantly increased (P˂0.001) egg production and egg mass. Alpha-tocopherol increased significantly (P = 0.002) only in the case of the 60 g/kg hempseed level (101 mg/kg dry matter (DM) versus 83 mg/ kg DM in the control group). In contrast, the level of γ-tocopherol increased gradually from 11 mg/kg DM in the control to 29, 39 and 43 mg/kg DM at the 30, 60 and 90 g/kg levels of dietary hempseed, respectively. The concentrations of beta carotene, zeaxanthin and lutein in egg yolks were not influenced by the dietary treatment. Hempseed at 90 g/kg (P = 0.036) decreased egg shell thickness without affecting its strength. The addition of hempseed decreased (P˂0.001) the cholesterol concentration in the egg yolks in all experimental groups. The addition of 30, 60 and 90 g hempseed increased (P˂0.001) the breaking strength of the tibia to 354, 352 and 350 N, respectively, compared to 297 N in the control group. The highest level of hempseed in the diet positively influenced the Ca concentration in the tibia (P = 0.021). The concentration of P in the tibia was negatively affected in the 60 to 90 g/kg hempseed treatments (P˂0.001). Eggs are a significant source of α-tocopherol. Based on our results, there is a possibility for enrichment of egg yolks with γ-tocopherol, with all of its associated health benefits, by the addition of hempseed to the diet of laying hens. Another significant benefit of hempseed is its effect on the breaking strength of the tibia, which can help with crucial problems in the commercial breeding of laying hens.

Department of Nutritional Physiology and Animal Product Quality Institute of Animal Science Prague Uhrineves Czech Republic

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Leger CL. Vitamin E: current state of knowledge, role in the prevention of cardiovascular disease, bioavailability. OCL-Oleagineux Corps Gras Lipides. 2000; 7: 258–265.

Kriese U, Schumann E, Weber WE, Bayer M., Brühl L, Matthāus B. Oil content, tocopherol composition and fatty acid patterns of the seeds of 51 Cannabis sativa L. genotypes. Euphytica. 2004; 137: 339–351.

Oomah BD, Busson M, Godfey GD, Drower JCD. Characteristics of hemp (Cannabis sativa L.) seed oil. Food Chem. 2002; 76: 33–43.

Jiang Q, Christen S, Schigenava MK, Ames BN. γ-Tocopherol, the major form of vitamin E in the USA diet, deserves more attention. Am. J. Clin. Nutr. 2001; 74: 714–722. 10.1093/ajcn/74.6.714 PubMed DOI

Kamal-Eldin A, Appelqvis LA. The chemistry and antioxidant properties of tocopherols and tocotrienols. Lipids. 1996; 31: 671–701. PubMed

Cooney RV, Franke AA, Harwood PJ, Hatch-Pigott V, Custer LJ, Mordan LJ. γ-Tocopherol detoxification of nitrogen dioxide: superiority to α-tocopherol. Proceedings of the National Academy of Sciences of the United States of America. 1993; 90: 1771–1775. 10.1073/pnas.90.5.1771 PubMed DOI PMC

Chin KY, Ima-Nirwana S. The Effects of α-Tocopherol on Bone: A Double-Edged Sword? Nutrients. 2014; 6: 1424–1441. 10.3390/nu6041424 PubMed DOI PMC

Kasai S, Ito A, Shindo K, Toyoshi T, Bando M. High-Dose α-Tocopherol Supplementation Does Not Induce Bone Loss in Normal Rats. PLoS ONE. 2015; 10: e0132059 10.1371/journal.pone.0132059 PubMed DOI PMC

Borhanuddin B, Fozi NFM, Mohamed IN. Vitamin E and the healing of bone fracture: The current state of evidence. Evid.-based Complement Altern. Med. 2012; Article ID 684510. PubMed PMC

Hamidi MS, Corey PN, Cheung AM. Effect of vitamin E on bone turnover markers among US postmenopausal women. J. Bone Miner. Res. 2012; 27: 1368–1380. 10.1002/jbmr.1566 PubMed DOI

Gabet Y. Cannabidiol enhances fracture healing by targeting collagen crosslinking. Osteroporosis Int. 2017; 28: S124.

Sazmand M, Mehrabani D, Hosseini SE, Amini M. The effect of hydroalcoholic extract of Cannabis Sativa on morphology and growth of bone marrow mesenchymal stem cells in rat. Electron. J. Gen. Med. 2018; 15: em32 10.29333/ejgm/86195. DOI

Goldberg EM, Gaghar N, Ryland D, Allani M, Gibson RA, House JD. Fatty acid profile and sensory characteristics of table eggs from laying hen fed hempseed and hempseed oil. J. Food Sci. 2012; 77: 153–160. PubMed

Neijat M, Gaghar N, Neufeld J, House JD. Performance, egg quality, and blood plasma chemistry of laying hens fed hempseed and hempseed oil. Poult. Sci. 2014; 93: 2827–2840. 10.3382/ps.2014-03936 PubMed DOI

Hale I, Schone F. Influence of rapeseed cake, linseed cake and hemp seed cake on laying performance of hens and fatty acid composition of egg yolk. J. Verbrauch. Lebensm. 2013; 8: 185–193.

Haugh RR. The Haugh unit for measuring egg quality. US Egg and Poultry Magazine. 1937; 43: 552–555, 572–573.

Sauveur B. Reproduction des volailles et production d’oeufs [Reproduction of poultry and egg production]. INRA, Paris; 1988. pp. 472.

Froescheis O, Moalli S, Liechti H, Bausch J. Determination of lycopene in tissues and plasma of rats by normal-phase high-performance liquid chromatography with photometric detection. J. Chromatogr. B. 2000; 739: 291–299. PubMed

SAS. SAS/STAT User’s Guide (release 9.3). Cary, NC, USA, SAS Institute; 2003.

Guinazi M, Milagres RCRM, Pinheiro-Sant´Ana HM, Chaves JBP. Tocopherols and tocotrienols in vegetable oils and eggs. Quim. Nova. 2009; 32: 2098–2103.

Gregory NG, Wilkins LJ. Broken bones in domestic fowl: handling and processing damage in end-of-lay battery hens. Br. Poult. Sci. 1989; 30: 555–562. 10.1080/00071668908417179 PubMed DOI

Budgell KL, Silversides FG. Bone breakage in three strains of end-of-lay hens. Can. J. Anim. Sci. 2004; 84: 745–747.

Dietary supplementation with xylanase suppresses the antinutritional effect of nonstarch polysaccharides of flaxseed and increases bone strength in broiler chickens

Effects of Dietary Hemp Seed and Flaxseed on Growth Performance, Meat Fatty Acid Compositions, Liver Tocopherol Concentration and Bone Strength of Cockerels