Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Antibody, Callose, Cell wall, Cellulose, Histochemistry, Lignin, Pectin, Staining, Suberin,
- MeSH
- Staining and Labeling methods MeSH
- Coloring Agents analysis MeSH
- Cell Wall chemistry ultrastructure MeSH
- Cellulose analysis MeSH
- Histocytochemistry methods MeSH
- Lignin analysis MeSH
- Lipids analysis MeSH
- Microscopy methods MeSH
- Pectins analysis MeSH
- Plants chemistry ultrastructure MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Coloring Agents MeSH
- Cellulose MeSH
- Lignin MeSH
- Lipids MeSH
- Pectins MeSH
Histochemical methods allow for identification and localization of various components within the tissue. Such information on the spatial heterogeneity is not available with biochemical methods. However, there is limitation of the specificity of such detection in context of complex tissue, which is important to consider, and interpretations of the results should regard suitable control treatments if possible. Such methods are valuable extension to specific optical and spectroscopic analytical methods. Here we present a set of selected simple methods of staining and histochemical tests with comments based on our laboratory experience.
Department of Experimental Plant Biology Faculty of Science Charles University Prague Czech Republic
References provided by Crossref.org
