Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Antibody, Callose, Cell wall, Cellulose, Histochemistry, Lignin, Pectin, Staining, Suberin,
- MeSH
- barvení a značení metody MeSH
- barvicí látky analýza MeSH
- buněčná stěna chemie ultrastruktura MeSH
- celulosa analýza MeSH
- histocytochemie metody MeSH
- lignin analýza MeSH
- lipidy analýza MeSH
- mikroskopie metody MeSH
- pektiny analýza MeSH
- rostliny chemie ultrastruktura MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- barvicí látky MeSH
- celulosa MeSH
- lignin MeSH
- lipidy MeSH
- pektiny MeSH
Histochemical methods allow for identification and localization of various components within the tissue. Such information on the spatial heterogeneity is not available with biochemical methods. However, there is limitation of the specificity of such detection in context of complex tissue, which is important to consider, and interpretations of the results should regard suitable control treatments if possible. Such methods are valuable extension to specific optical and spectroscopic analytical methods. Here we present a set of selected simple methods of staining and histochemical tests with comments based on our laboratory experience.
Department of Experimental Plant Biology Faculty of Science Charles University Prague Czech Republic
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